ArticleNucleic acids research2013
Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- A self-replicating artificial module-genome that generates bacterial chromosome replication system in vitro.Nucleic acids research · 2026Article
- Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method.Nature communications · 2025Article
- Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.Molecules (Basel, Switzerland) · 2024Article
- Noise Minimization in Cell-Free Gene Expression.ACS synthetic biology · 2023Article
- Progresses in Cell-Free In Vitro Evolution.Advances in biochemical engineering/biotechnology · 2023Review
- What remains from living cells in bacterial lysate-based cell-free systems.Computational and structural biotechnology journal · 2023Review
- Phospholipid synthesis inside phospholipid membrane vesicles.Communications biology · 2022Article
- The Pore-Forming Hemolysin BL Enterotoxin fromToxins · 2021Article
- Towards a synthetic cell cycle.Nature communications · 2021Review
- A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression.Life (Basel, Switzerland) · 2021Article
- Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus.Scientific reports · 2020Article
- In vitro self-replication and multicistronic expression of large synthetic genomes.Nature communications · 2020Article
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system.Chemical science · 2019Article
- Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System.Chembiochem : a European journal of chemical biology · 2019Article
- Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination.Scientific reports · 2018Article
- Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor.Scientific reports · 2018Article
- Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.Nature communications · 2018Article
- In vitro transcription-translation using bacterial genome as a template to reconstitute intracellular profile.Nucleic acids research · 2017Article
- Cell-Free Synthetic Biology: Engineering Beyond the Cell.Cold Spring Harbor perspectives in biology · 2016Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription-translation-replication process by autonomous expression of chromosomal DNA replication machineries from a reconstituted transcription-translation system (PURE system). We found that low temperature is essential to express a complex protein, DNA polymerase III, in a single tube using the PURE system. Addition of the 13 genes, encoding initiator, DNA helicase, helicase loader, RNA primase and DNA polymerase III to the PURE system gave rise to a DNA replication system by a coupling manner. An artificial genetic circuit demonstrated that the DNA produced as a result of the replication is able to provide genetic information for proteins, indicating the in vitro central dogma can sequentially undergo two rounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.