ArticleNucleic acids research2013
Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- A Review on Perception of Binding Kinetics in Affinity Biosensors: Challenges and Opportunities.ACS omega · 2025Review
- A scalable framework for the discovery of functional helicase substrates and helicase-driven regulatory switches.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Identification of nucleic acid aptamers against lactate dehydrogenase via SELEX and high-throughput sequencing.Analytical and bioanalytical chemistry · 2021Article
- Development of ssDNA Aptamers for Diagnosis and Inhibition of the Highly Pathogenic Avian Influenza Virus Subtype H5N1.Biomolecules · 2020Article
- Inside the Black Box: What Makes SELEX Better?Molecules (Basel, Switzerland) · 2019Review
- The impact of immobilized metal affinity chromatography (IMAC) resins on DNA aptamer selection.Analytical and bioanalytical chemistry · 2014Article
- New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.Molecular therapy. Nucleic acids · 2014Article
- High-throughput binding characterization of RNA aptamer selections using a microplate-based multiplex microcolumn device.Analytical and bioanalytical chemistry · 2014Article
- Discovering aptamers by cell-SELEX against human soluble growth factors ectopically expressed on yeast cell surface.PloS one · 2014Article
- RAPID-SELEX for RNA aptamers.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The non-specific binding of undesired ligands to a target is the primary factor limiting the enrichment of tight-binding ligands in affinity selection. Solution-phase non-specific affinity is determined by the free-energy of ligand binding to a single target. However, the solid-phase affinity might be higher if a ligand bound concurrently to multiple adjacent immobilized targets in a cooperative manner. Cooperativity could emerge in this case as a simple consequence of the relationship between the free energy of binding, localization entropy and the spatial distribution of the immobilized targets. We tested this hypothesis using a SELEX experimental design and found that non-specific RNA aptamer ligands can concurrently bind up to four bead-immobilized peptide targets, and that this can increase their effective binding affinity by two orders-of-magnitude. Binding curves were quantitatively explained by a new statistical mechanical model of density-dependent cooperative binding, which relates cooperative binding to both the target concentration and the target surface density on the immobilizing substrate. Target immobilization plays a key role in SELEX and other ligand enrichment methods, particularly in new multiplexed microfluidic purification devices, and these results have strong implications for optimizing their performance.
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Registered trials
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