ArticleBlood transfusion = Trasfusione del sangue2014
A new strategy to identify rare blood donors: single polymerase chain reaction multiplex SNaPshot reaction for detection of 16 blood group alleles.
Article in Blood transfusion = Trasfusione del sangue, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Blood Group Testing.Frontiers in medicine · 2022Review
- Molecular Detection of Insecticide Resistance Mutations inFrontiers in cellular and infection microbiology · 2021Article
- Blood groups in Native Americans: a look beyond ABO and Rh.Genetics and molecular biology · 2021Article
- Impact of using genotyping to predict SERF negative phenotype in Thai blood donor populations.Blood research · 2020Article
- Cystic Fibrosis: A Simple and Customized Strategy for Genetic Screening Able to Detect Over 90% of Identified Mutated Alleles in Brazilian Newborns.Molecular diagnosis & therapy · 2020Article
- SMIM1 polymorphisms in a donor population from southeast Brazil and their correlation with VEL expression.Blood transfusion = Trasfusione del sangue · 2019Article
- The Impact of Using Genotyped Reagent Red Blood Cells in Antibody Identification.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2018Article
- Red cell genotyping precision medicine: a conference summary.Therapeutic advances in hematology · 2017Review
- Evolution of technology for molecular genotyping in blood group systems.The Indian journal of medical research · 2017Review
- Molecular immunohaematology round table discussions at the AABB Annual Meeting, Philadelphia 2014.Blood transfusion = Trasfusione del sangue · 2016Article
- Implementing mass-scale red cell genotyping at a blood center.Transfusion · 2015Article
- Rare blood donors screening by multiplex PCR methods in Chinese Zhuang and Dong population and pedigree analysis.International journal of clinical and experimental medicine · 2015Article
- Frequency of Wr(a) antigen and anti-Wr(a) in Brazilian blood donors.Revista brasileira de hematologia e hemoterapiaArticle
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs an alternative to phenotyping, large-scale DNA-based assays, which are feasible for high-throughput donor red blood cell typing, were developed for determination of blood group polymorphisms. However, high-throughput genotyping platforms based on these technologies are still expensive and the inclusion of single nucleotide polymorphisms and analysis of the alleles depend on the manufacturer's determination. To overcome this limitation and in order to develop an assay to enable the screening of rare donors, we developed a SNaPshot assay for analysis of nine single nucleotide polymorphisms related to antigens that are difficult to assess using conventional serology. MATERIALS AND
methodsThe single polymerase chain reaction multiplex SNaPshot reaction was optimized to identify nine single nucleotide polymorphisms determining 16 alleles: KEL*3/KEL*4, KEL*6/KEL*7, DI*1/DI*2, DI*3/DI*4, YT*1/YT*2, CO*1/CO*2, DO*1/DO*2, DO*4, DO*5. We designed a single multiplex PCR with primers encompassing the blood group single nucleotide polymorphisms and performed an internal reaction with probe primers able to discriminate the alleles after fragment analysis. The SNaPshot assay was validated with 140 known alleles previously determined by PCR restriction fragment length polymorphism.
resultsWe were able to simultaneous detect nine single nucleotide polymorphisms defining 16 blood group alleles on an assay based on a multiplex PCR combined with a single base extension using genomic DNA. DISCUSSION: This study demonstrates a robust genotyping strategy for conducting rare donor screening which can be applied in blood centers and could be an important tool for identifying antigen-negative donors and, therefore, for providing rare blood.
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