ArticlePlant cell reports2013
Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins.BMC plant biology · 2025Article
- Morphological and Transcriptomic Analyses Reveal the Involvement of Key Metabolic Pathways in Male Sterility inPlants (Basel, Switzerland) · 2024Article
- Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat.Frontiers in plant science · 2022Article
- Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- Embryogenesis of European Radish (Plants (Basel, Switzerland) · 2021Article
- Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.Plant reproduction · 2020Article
- Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.Journal of experimental botany · 2019Article
- Transcriptome and Hormone Comparison of Three Cytoplasmic Male Sterile Systems inInternational journal of molecular sciences · 2018Article
- Microspore embryogenesis in Brassica: calcium signaling, epigenetic modification, and programmed cell death.Planta · 2018Review
- The functional analysis of SlNCED1 in tomato pollen development.Cellular and molecular life sciences : CMLS · 2018Article
- A Specialized Histone H1 Variant Is Required for Adaptive Responses to Complex Abiotic Stress and Related DNA Methylation in Arabidopsis.Plant physiology · 2015Article
- Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures.Plant cell reports · 2015Article
- Current insights into hormonal regulation of microspore embryogenesis.Frontiers in plant science · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA better understanding of androgenesis with a focus on the changes in plasma membrane fluidity and endogenous ABA content affecting embryogenesis induction in microspore suspension of B. napus. Changes in plasma membrane fluidity (MF) and ABA content associated with androgenesis induction were under the study. Both parameters were monitored in microspores of two Brassica napus L. genotypes differing in their response to androgenic induction under heat (1 day at 32 °C). MF was assessed by DPH method. ABA content was evaluated by ELISA. Heat caused microspores' plasma membrane to become more rigid. Lower MF in microspores of 'DH 4079' (of high androgenic potential) seems to maintain proper cell protection and leads to efficient embryogenesis induction. Plasma membrane remodelling coincided with changes of ABA content in microspores and in the culture medium in both genotypes. ABA concentration (μM) and ABA content (fmol per 10(4) microspores or pmol g(-1) FW) were for the first time measured in microspores. ABA concentration (μM) in microspores and in the culture medium (nM) differed significantly for the genotype and the treatment. The interaction between both variables was also significant. In general, ABA content ranged from <3.5 to 87.1 fmol per 10(4) microspores. The highest content of ABA was detected in 'DH 4079' microspores at 32 °C. Assuming a mean microspores' radius of 10 μm, it corresponds to ABA concentration of 2.1 μM. Heat shock resulted in quantum of medium pH reduction (0.1-0.2) and increased levels of ABA in microspores and in the medium of both tested genotypes. However, heat induced increase of ABA content in microspores of non-responsive 'Campino' had no clear-cut impact, on androgenesis induction efficiency, which suggests a more complex mechanism of process initiation.
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