ArticlePloS one2013
Detection of osteogenic differentiation by differential mineralized matrix production in mesenchymal stromal cells by Raman spectroscopy.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Exploring In Vitro Mesenchymal Stem Cell Osteodifferentiation via Vibrational Microspectroscopy: A Review.Stem cell reviews and reports · 2025Review
- Osteoinductive potential of graphene and graphene oxide for bone tissue engineering: a comparative study.Journal of orthopaedic surgery and research · 2024Article
- Neural regeneration ability of Polypyrrole-Collagen-Quercetin composite in the spinal cord injury.Regenerative therapy · 2023Article
- Label-free optical imaging and sensing for quality control of stem cell manufacturing.Current opinion in biomedical engineering · 2023Article
- The emerging applications and advancements of Raman spectroscopy in pediatric cancers.Frontiers in oncology · 2023Review
- Design of a custom-made device for real-time optical measurement of differential mineral concentrations in three-dimensional scaffolds for bone tissue engineering.Royal Society open science · 2022Article
- Compositional assessment of bone by Raman spectroscopy.The Analyst · 2021Review
- A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential.Matrix biology plus · 2020Article
- Bone quality assessment of osteogenic cell cultures by Raman microscopy.Journal of Raman spectroscopy : JRS · 2019Article
- Osteocalcin Mediates Biomineralization during Osteogenic Maturation in Human Mesenchymal Stromal Cells.International journal of molecular sciences · 2017Article
- Non-invasive Raman Spectroscopy and Quantitative Real-Time PCR Distinguish Among Undifferentiated Human Mesenchymal Stem Cells and Redifferentiated Nucleus Pulposus Cells and ChondrocytesThe open biomedical engineering journal · 2017Article
- Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization.Stem cells international · 2017Article
- Label-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells.Scientific reports · 2016Article
- Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration.Journal of the Royal Society, Interface · 2016Review
- Detection of hepatic maturation by Raman spectroscopy in mesenchymal stromal cells undergoing hepatic differentiation.Stem cell research & therapy · 2016Article
- Identifying States along the Hematopoietic Stem Cell Differentiation Hierarchy with Single Cell Specificity via Raman Spectroscopy.Analytical chemistry · 2015Article
- Aseptic Raman spectroscopy can detect changes associated with the culture of human dental pulp stromal cells in osteoinductive culture.The Analyst · 2015Article
- Raman spectroscopy for grading of live osteosarcoma cells.Stem cell research & therapy · 2015Article
- Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications.Current opinion in biotechnology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stromal cells (MSCs) hold great potential in skeletal tissue engineering and regenerative medicine. However, conventional methods that are used in molecular biology to evaluate osteogenic differentiation of MSCs require a relatively large amount of cells. Cell lysis and cell fixation are also required and all these steps are time-consuming. Therefore, it is imperative to develop a facile technique which can provide real-time information with high sensitivity and selectivity to detect the osteogenic maturation of MSCs. In this study, we use Raman spectroscopy as a biosensor to monitor the production of mineralized matrices during osteogenic induction of MSCs. In summary, Raman spectroscopy is an excellent biosensor to detect the extent of maturation level during MSCs-osteoblast differentiation with a non-disruptive, real-time and label free manner. We expect that this study will promote further investigation of stem cell research and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.