ArticlePloS one2013
Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 100 citations in OpenAlex.
- Review
- Targeted disruption of vacuolar-sorting protein and inhibitor of apoptosis genes via virus-induced gene silencing alters survival and reproduction of Diaphorina citri.Pest management science · 2026Article
- In Planta system-mediated double-stranded RNA delivery targeting Laccase 2 and Tyrosine 3-monooxygenase genes disrupt Diaphorina citri development.Insect science · 2026Article
- Syntaxin-1A Silencing by RNAi Disrupts Growth and Reproduction in the Asian Citrus Psyllid,Insects · 2025Article
- Virus-induced gene silencing simultaneously exploits 'attract and kill' traits in plants and insects to manage huanglongbing.Horticulture research · 2025Article
- Article
- Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae).Journal of insect science (Online) · 2023Article
- RNA Interference Past and Future Applications in Plants.International journal of molecular sciences · 2023Review
- Gene silencing of cathepsins B and L using CTV-based, plant-mediated RNAi interferes with ovarial development in Asian citrus psyllid (ACP),Frontiers in plant science · 2023Article
- Differential gene expression of Asian citrus psyllids infected with 'Frontiers in plant science · 2023Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications.International journal of molecular sciences · 2022Review
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Characteristics of environmental RNAi in potato psyllid,Frontiers in physiology · 2022Article
- The Chorion Proteome ofInsects · 2021Article
- Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid,Insects · 2021Article
- Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers.Plants (Basel, Switzerland) · 2021Article
- Crowdsourced Identification of Potential Target Genes for CTV Induced Gene Silencing for Controlling the Citrus Greening VectorFrontiers in physiology · 2021Article
- Article
- Exploiting somatic piRNAs inLife science alliance · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (awd) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of awd is development-dependent and the highest level was found in the last instar (5(th)) of the nymphal stage. Micro-application (topical application) of dsRNA to 5(th) instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the awd gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-gfp was used, indicating the specific effect of dsRNA-awd. Our findings suggest a role for awd in ACP wing development and metamorphosis. awd could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA-awd. These strategies will help to mitigate HLB by controlling ACP.
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