ArticlePloS one2013
To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mental health dished up-the use of iPSC models in neuropsychiatric research.Journal of neural transmission (Vienna, Austria : 1996) · 2020Pooled it
- Simple and efficient differentiation of human iPSCs into contractible skeletal muscles for muscular disease modeling.Scientific reports · 2023Article
- Epigenetic biomarkers to track differentiation of pluripotent stem cells.Stem cell reports · 2023Article
- Tumor suppressors inhibit reprogramming of African spiny mouse (Wellcome open research · 2022Article
- Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells.Regenerative biomaterials · 2022Article
- Novel test strategies for in vitro seizure liability assessment.Expert opinion on drug metabolism & toxicology · 2021Review
- PRDM8 reveals aberrant DNA methylation in aging syndromes and is relevant for hematopoietic and neuronal differentiation.Clinical epigenetics · 2020Article
- Genetic barcoding reveals clonal dominance in iPSC-derived mesenchymal stromal cells.Stem cell research & therapy · 2020Article
- Senescence-Associated Metabolomic Phenotype in Primary and iPSC-Derived Mesenchymal Stromal Cells.Stem cell reports · 2020Article
- The StemCellFactory: A Modular System Integration for Automated Generation and Expansion of Human Induced Pluripotent Stem Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications.Cell transplantation · 2019Review
- Differentiation of Induced Pluripotent Stem Cells towards Mesenchymal Stromal Cells is Hampered by Culture in 3D Hydrogels.Scientific reports · 2019Article
- Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells.Clinical epigenetics · 2019Article
- Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells.Scientific reports · 2018Article
- Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies.Stem cell reports · 2017Article
- Human Induced Pluripotent Stem Cells as a Platform for Personalized and Precision Cardiovascular Medicine.Physiological reviews · 2016Review
- Advancing drug discovery for neuropsychiatric disorders using patient-specific stem cell models.Molecular and cellular neurosciences · 2016Review
- Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells.Scientific reports · 2016Article
- A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.Stem cells translational medicine · 2015Article
- Epigenetic biomarker to support classification into pluripotent and non-pluripotent cells.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induced pluripotent stem cells (iPSCs) are usually clonally derived. The selection of fully reprogrammed cells generally involves picking of individual colonies with morphology similar to embryonic stem cells (ESCs). Given that fully reprogrammed cells are highly proliferative and escape from cellular senescence, it is conceivable that they outgrow non-pluripotent and partially reprogrammed cells during culture expansion without the need of clonal selection. In this study, we have reprogrammed human dermal fibroblasts (HDFs) with episomal plasmid vectors. Colony frequency was higher and size was larger when using murine embryonic fibroblasts (MEFs) as stromal support instead of HDFs or human mesenchymal stromal cells (MSCs). We have then compared iPSCs which were either clonally derived by manual selection of a single colony, or derived from bulk-cultures of all initial colonies. After few passages their morphology, expression of pluripotency markers, and gene expression profiles did not reveal any significant differences. Furthermore, clonally-derived and bulk-cultured iPSCs revealed similar in vitro differentiation potential towards the three germ layers. Therefore, manual selection of individual colonies does not appear to be necessary for the generation of iPSCs - this is of relevance for standardization and automation of cell culture procedures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.