ArticlePloS one2013
Transcriptome and proteome data reveal candidate genes for pollinator attraction in sexually deceptive orchids.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Orchid genome evolution and trait innovation.Journal of integrative plant biology · 2026Review
- Genome Assemblies of the Daisy Gorteria diffusa Elucidate the Molecular-Genomic Basis of Pollination by Sexual Deception.Genome biology and evolution · 2026Article
- Transcriptome characterization and population genetics ofBiodiversity data journal · 2026Article
- Plants, Pollinators and Pheromones: Promises and Lies of Semiochemicals.Plant, cell & environment · 2025Review
- Fine-tuned terpene synthase gene expression, functional promiscuity, and subcellular localization: implications for the evolution of complex floral volatile bouquet in Caladenia orchids.Plant & cell physiology · 2025Article
- Genome of the early spider-orchid Ophrys sphegodes provides insights into sexual deception and pollinator adaptation.Nature communications · 2024Article
- Morphological Continua Make Poor Species: Genus-Wide Morphometric Survey of the European Bee Orchids (Biology · 2023Article
- Orchid Phylotranscriptomics: The Prospects of Repurposing Multi-Tissue Transcriptomes for Phylogenetic Analysis and Beyond.Frontiers in plant science · 2022Article
- A Walk Through the Maze of Secondary Metabolism in Orchids: A Transcriptomic Approach.Frontiers in plant science · 2022Review
- Anthocyanin and Flavonol Glycoside Metabolic Pathways Underpin Floral Color Mimicry and Contrast in a Sexually Deceptive Orchid.Frontiers in plant science · 2022Article
- Review
- Detection of superoxide dismutase (Cu-Zn) isoenzymes in leaves and pseudobulbs ofBiochemistry and biophysics reports · 2020Article
- Identification of (International journal of molecular sciences · 2020Article
- Duplication and selection in β-ketoacyl-ACP synthase gene lineages in the sexually deceptive Chiloglottis (Orchidaceace).Annals of botany · 2019Article
- Article
- A Phylogenomic Analysis of the Floral Transcriptomes of Sexually Deceptive and Rewarding European Orchids,Frontiers in plant science · 2019Article
- Comparative transcriptomics provides insight into the molecular basis of species diversification of section Trigonopedia (Cypripedium) on the Qinghai-Tibetan Plateau.Scientific reports · 2018Article
- Integrating restriction site-associated DNA sequencing (RAD-seq) with morphological cladistic analysis clarifies evolutionary relationships among major species groups of bee orchids.Annals of botany · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- Transcriptome-Wide Identification and Expression Analysis of DIVARICATA- and RADIALIS-Like Genes of the Mediterranean Orchid Orchis italica.Genome biology and evolution · 2017Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSexually deceptive orchids of the genus Ophrys mimic the mating signals of their pollinator females to attract males as pollinators. This mode of pollination is highly specific and leads to strong reproductive isolation between species. This study aims to identify candidate genes responsible for pollinator attraction and reproductive isolation between three closely related species, O. exaltata, O. sphegodes and O. garganica. Floral traits such as odour, colour and morphology are necessary for successful pollinator attraction. In particular, different odour hydrocarbon profiles have been linked to differences in specific pollinator attraction among these species. Therefore, the identification of genes involved in these traits is important for understanding the molecular basis of pollinator attraction by sexually deceptive orchids.
resultsWe have created floral reference transcriptomes and proteomes for these three Ophrys species using a combination of next-generation sequencing (454 and Solexa), Sanger sequencing, and shotgun proteomics (tandem mass spectrometry). In total, 121 917 unique transcripts and 3531 proteins were identified. This represents the first orchid proteome and transcriptome from the orchid subfamily Orchidoideae. Proteome data revealed proteins corresponding to 2644 transcripts and 887 proteins not observed in the transcriptome. Candidate genes for hydrocarbon and anthocyanin biosynthesis were represented by 156 and 61 unique transcripts in 20 and 7 genes classes, respectively. Moreover, transcription factors putatively involved in the regulation of flower odour, colour and morphology were annotated, including Myb, MADS and TCP factors.
conclusionOur comprehensive data set generated by combining transcriptome and proteome technologies allowed identification of candidate genes for pollinator attraction and reproductive isolation among sexually deceptive orchids. This includes genes for hydrocarbon and anthocyanin biosynthesis and regulation, and the development of floral morphology. These data will serve as an invaluable resource for research in orchid floral biology, enabling studies into the molecular mechanisms of pollinator attraction and speciation.
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