ArticlePloS one2013
cell- and tissue-specific transcriptome analyses of Medicago truncatula root nodules.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed.
- Single-Cell RNA-Sequencing in Nodulated Roots of Medicago truncatula.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Molecular mechanisms modulating beneficial plant root-microbe interactions: What's common?Plant communications · 2026Review
- Single-Cell Omics in Legumes: Research Trends and Applications.Plants (Basel, Switzerland) · 2025Review
- Identification and functional evaluation of cyclin-dependent kinase genes reveals that CDKB1;1 and CDKB2;2 contribute to the balance of mitosis and endoreduplication in Medicago truncatula nodule.BMC plant biology · 2025Article
- Gibberellin dynamics governing nodulation revealed using GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula lateral organs.The Plant cell · 2024Article
- Mapping the molecular landscape of Lotus japonicus nodule organogenesis through spatiotemporal transcriptomics.Nature communications · 2024Article
- Laser Capture Microdissection Transcriptome Reveals Spatiotemporal Tissue Gene Expression Patterns ofMolecular plant-microbe interactions : MPMI · 2023Article
- Expression and mutagenesis studies in theFrontiers in plant science · 2023Article
- Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules ofInternational journal of molecular sciences · 2022Article
- To have or not to have: expression of amino acid transporters during pathogen infection.Plant molecular biology · 2022Review
- DNA demethylation and hypermethylation are both required for late nodule development in Medicago.Nature plants · 2022Article
- Reference nodule transcriptomes forPlant direct · 2022Article
- Cleaning thePlants (Basel, Switzerland) · 2021Article
- Review
- Article
- The Medicago truncatula Vacuolar iron Transporter-Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process.The New phytologist · 2020Article
- Reprogramming of Root Cells during Nitrogen-Fixing Symbiosis Involves Dynamic Polysome Association of Coding and Noncoding RNAs.The Plant cell · 2020Article
- A Roadmap toward Engineered Nitrogen-Fixing Nodule Symbiosis.Plant communications · 2020Review
- Gene Expression in Nitrogen-Fixing Symbiotic Nodule Cells inThe Plant cell · 2020Review
- An optimized protocol for isolation of high-quality RNA through laser capture microdissection of leaf material.Plant direct · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture microdissection. Next, we determined their associated expression profiles using Affymetrix Medicago GeneChips. Cells were collected from the nodule infection zone divided into a distal (where symbiosome formation and division occur) and proximal region (where symbiosomes are mainly differentiating), as well as infected cells from the fixation zone containing mature nitrogen fixing symbiosomes. As non-infected cells/tissue we included nodule meristem cells and uninfected cells from the fixation zone. Here, we present a comprehensive gene expression map of an indeterminate Medicago nodule and selected genes that show specific enriched expression in the different cells or tissues. Validation of the obtained expression profiles, by comparison to published gene expression profiles and experimental verification, indicates that the data can be used as digital "in situ". This digital "in situ" offers a genome-wide insight into genes specifically associated with subsequent stages of symbiosome and nodule cell development, and can serve to guide future functional studies.
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