ArticleFrontiers in molecular neuroscience2013
Transgenic mouse lines for non-invasive ratiometric monitoring of intracellular chloride.
Article in Frontiers in molecular neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- A humanized chemogenetic system inhibits murine pain-related behavior and hyperactivity in human sensory neurons.Science translational medicine · 2023Article
- Long-Term Imaging Reveals a Circadian Rhythm of Intracellular Chloride in Neurons of the Suprachiasmatic Nucleus.Journal of biological rhythms · 2022Article
- Improving NKCC1 Function Increases the Excitability of DRG Neurons Exacerbating Pain Induced After TRPV1 Activation of Primary Sensory Neurons.Frontiers in cellular neuroscience · 2021Article
- Distal convoluted tubule ClAmerican journal of physiology. Renal physiology · 2020Article
- Expression and effect of sodium-potassium-chloride cotransporter on dorsal root ganglion neurons in a rat model of chronic constriction injury.Neural regeneration research · 2020Article
- Neuroprotection by Propofol Post-Conditioning: Focus on PKMζ/KCC2 Pathway Activity.Cellular and molecular neurobiology · 2018Article
- Intracellular Chloride Regulation in AVP+ and VIP+ Neurons of the Suprachiasmatic Nucleus.Scientific reports · 2017Article
- A noninvasive optical approach for assessing chloride extrusion activity of the K-Cl cotransporter KCC2 in neuronal cells.BMC neuroscience · 2017Article
- A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence.Science advances · 2016Article
- TMEM16F Regulates Spinal Microglial Function in Neuropathic Pain States.Cell reports · 2016Article
- Current view on the functional regulation of the neuronal K(+)-Cl(-) cotransporter KCC2.Frontiers in cellular neuroscience · 2014Review
- Correlative intravital imaging of cGMP signals and vasodilation in mice.Frontiers in physiology · 2014Article
- Twenty years of fluorescence imaging of intracellular chloride.Frontiers in cellular neuroscience · 2014Review
- Tools, methods, and applications for optophysiology in neuroscience.Frontiers in molecular neuroscience · 2013Article
- Molecular probes and switches for functional analysis of receptors, ion channels and synaptic networks.Frontiers in molecular neuroscience · 2013Article
- Calibration and functional analysis of three genetically encoded Cl(-)/pH sensors.Frontiers in molecular neuroscience · 2013Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloride is the most abundant physiological anion and participates in a variety of cellular processes including trans-epithelial transport, cell volume regulation, and regulation of electrical excitability. The development of tools to monitor intracellular chloride concentration ([Cli]) is therefore important for the evaluation of cellular function in normal and pathological conditions. Recently, several Cl-sensitive genetically encoded probes have been described which allow for non-invasive monitoring of [Cli]. Here we describe two mouse lines expressing a CFP-YFP-based Cl probe called Cl-Sensor. First, we generated transgenic mice expressing Cl-Sensor under the control of the mouse Thy1 mini promoter. Cl-Sensor exhibited good expression from postnatal day two (P2) in neurons of the hippocampus and cortex, and its level increased strongly during development. Using simultaneous whole-cell monitoring of ionic currents and Cl-dependent fluorescence, we determined that the apparent EC 50 for Cli was 46 mM, indicating that this line is appropriate for measuring neuronal [Cli] in postnatal mice. We also describe a transgenic mouse reporter line for Cre-dependent conditional expression of Cl-Sensor, which was targeted to the Rosa26 locus and by incorporating a strong exogenous promoter induced robust expression upon Cre-mediated recombination. We demonstrate high levels of tissue-specific expression in two different Cre-driver lines targeting cells of the myeloid lineage and peripheral sensory neurons. Using these mice the apparent EC 50 for Cli was estimated to be 61 and 54 mM in macrophages and DRG, respectively. Our data suggest that these mouse lines will be useful models for ratiometric monitoring of Cli in specific cell types in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.