ArticleStem cells translational medicine2013
Engineering a blood-retinal barrier with human embryonic stem cell-derived retinal pigment epithelium: transcriptome and functional analysis.
Article in Stem cells translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Integrated transcriptomic and metabolomic analyses reveal distinct energy metabolic signatures and functional properties of RPE cells under two culture conditions.Scientific reports · 2026Article
- Regulation of RPE65 expression in human retinal pigment epithelium cells.Scientific reports · 2025Article
- Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy.Bioactive materials · 2024Review
- Human pluripotent stem cells for the modelling of retinal pigment epithelium homeostasis and disease: A review.Clinical & experimental ophthalmology · 2022Review
- Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration.Stem cells translational medicine · 2022Review
- Chromosome 10q26-driven age-related macular degeneration is associated with reduced levels ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Let-7, Lin28 and Hmga2 Expression in Ciliary Epithelium and Retinal Progenitor Cells.Investigative ophthalmology & visual science · 2021Article
- Knockdown of Claudin-19 in the Retinal Pigment Epithelium Is Accompanied by Slowed Phagocytosis and Increased Expression of SQSTM1.Investigative ophthalmology & visual science · 2021Article
- Coculture techniques for modeling retinal development and disease, and enabling regenerative medicine.Stem cells translational medicine · 2020Review
- Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium.Investigative ophthalmology & visual science · 2020Article
- Retinal Tissue Bioengineering, Materials and Methods for the Treatment of Glaucoma.Tissue engineering and regenerative medicine · 2020Review
- Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions.Stem cells translational medicine · 2020Article
- Eye in a Disk: eyeIntegration Human Pan-Eye and Body Transcriptome Database Version 1.0.Investigative ophthalmology & visual science · 2019Article
- Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.Stem cells translational medicine · 2019Article
- Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function.Communications biology · 2019Article
- Identifying core biological processes distinguishing human eye tissues with precise systems-level gene expression analyses and weighted correlation networks.Human molecular genetics · 2018Article
- Cell-based therapeutic strategies for replacement and preservation in retinal degenerative diseases.Progress in retinal and eye research · 2017Review
- RNA-Sequencing data supports the existence of novel VEGFA splicing events but not of VEGFAScientific reports · 2017Article
- Characterization of lincRNA expression in the human retinal pigment epithelium and differentiated induced pluripotent stem cells.PloS one · 2017Article
- Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells.Molecular vision · 2017Article
Corrections and comments
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retinal degenerations are a major cause of impaired vision in the elderly. Degenerations originate in either photoreceptors or the retinal pigment epithelium (RPE). RPE forms the outer blood-retinal barrier and functions intimately with photoreceptors. Animal models and cultures of RPE are commonly used to screen potential pharmaceuticals or explore RPE replacement therapy, but human RPE differs from that of other species. Human RPE forms a barrier using tight junctions composed of a unique set of claudins, proteins that determine the permeability and selectivity of tight junctions. Human adult RPE fails to replicate these properties in vitro. To develop a culture model for drug development and tissue-engineering human retina, RPE were derived from human embryonic stem cells (hESCs). Barrier properties of RPE derived from the H1 and H9 hESC lines were compared with a well-regarded model of RPE function, human fetal RPE isolated from 16-week-gestation fetuses (hfRPE). A serum-free medium (SFM-1) that enhanced the redifferentiation of hfRPE in culture also furthered the maturation of hESC-derived RPE. In SFM-1, the composition, selectivity, and permeability of tight junctions were similar to those of hfRPE. Comparison of the transcriptomes by RNA sequencing and quantitative reverse transcription-polymerase chain reaction revealed a high correlation between the hESCs and hfRPE, but there were notable differences in the expression of adhesion junction and membrane transport genes. These data indicated that hESC-derived RPE is highly differentiated but may be less mature than RPE isolated from 16-week fetuses. The study identified a panel of genes to monitor the maturation of RPE.
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