ArticleProceedings of the National Academy of Sciences of the United States of America2013
Wolbachia uses a host microRNA to regulate transcripts of a methyltransferase, contributing to dengue virus inhibition in Aedes aegypti.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers, 1 of them a synthesis that pooled it.
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Who cites it
122 citing papers in PubMed, 1 synthesis or guideline pooled it.
- microRNA profiles and functions in mosquitoes.PLoS neglected tropical diseases · 2018Pooled it
- Microbial epigenetic regulation as a multilevel regulatory interface in host-microbe interactions.Gut microbes · 2026Review
- Microbiome-Epigenome Interplay Impacts Microbial Symbiosis and Stress Adaptations in the Brown Planthopper (International journal of molecular sciences · 2026Article
- Unlocking new frontiers in vector control strategies using Aedes aegypti microbiota.Parasites & vectors · 2026Review
- Article
- Wolbachia-driven host miRNAs mediate arthropod reproduction in a Wolbachia density-dependent manner.Communications biology · 2026Article
- The inhibition mechanism ofFrontiers in microbiology · 2026Review
- Selected gut bacteria promote mosquito larval growth through the regulation of ionic microenvironment and protease expression.iScience · 2025Article
- Wolbachia elevates host methyltransferase expression and alters the mBMC microbiology · 2025Article
- The infectivity of virus particles from Wolbachia-infected Drosophila.BMC microbiology · 2025Article
- Understanding the role of trehalose in interactions betweenFrontiers in cellular and infection microbiology · 2025Review
- Generating prophylactic immunity against arboviruses in vertebrates and invertebrates.Nature reviews. Immunology · 2024Review
- Wolbachia mediates crosstalk between miRNA and Toll pathways to enhance resistance to dengue virus in Aedes aegypti.PLoS pathogens · 2024Article
- Effects of Arboviral Infections on Transposable Element Transcript Levels in Aedes aegypti.Genome biology and evolution · 2024Article
- AntiviralmBio · 2024Article
- A comprehensive review ofFrontiers in immunology · 2024Review
- Viral Prevalence and Genomic Xenology in the Coevolution of HzNV-2 (Nudiviridae) with HostInsects · 2023Article
- Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease.Scientific reports · 2023Article
- Holobiont perspectives on tripartite interactions among microbiota, mosquitoes, and pathogens.The ISME journal · 2023Review
- Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response.Scientific reports · 2023Article
62 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endosymbiont Wolbachia is common among insects and known for the reproductive manipulations it exerts on hosts as well as inhibition of virus replication in their hosts. Recently, we showed that Wolbachia uses host microRNAs to manipulate host gene expression for its efficient maintenance in the dengue mosquito vector, Aedes aegypti. Cytosine methylation is mediated by a group of proteins called DNA (cytosine-5) methyltransferases, which are structurally and functionally conserved from prokaryotes to eukaryotes. The biological functions of cytosine methylation include host defense, genome stability, gene regulation, developmental promotion of organs, and lifespan regulation. Ae. aegypti has only one DNA methyltransferase gene (AaDnmt2) belonging to the cytosine methyltransferase family 2, which is the most deeply conserved and widely distributed gene among metazoans. Here, we show that in mosquitoes the introduced endosymbiont, Wolbachia, significantly suppresses expression of AaDnmt2, but dengue virus induces expression of AaDnmt2. Interestingly, we found that aae-miR-2940 microRNA, which is exclusively expressed in Wolbachia-infected mosquitoes, down-regulates the expression of AaDnmt2. Reversely, overexpression of AaDnmt2 in mosquito cells led to inhibition of Wolbachia replication, but significantly promoted replication of dengue virus, suggesting a causal link between this Wolbachia manipulation and the blocking of dengue replication in Wolbachia-infected mosquitoes. In addition, our findings provide an explanation for hypomethylation of the genome in Wolbachia-infected Ae. aegypti.
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