ArticleProceedings of the National Academy of Sciences of the United States of America2013
Arabidopsis μ-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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80 citing papers in PubMed, 148 citations in OpenAlex.
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- The role of clathrin in post-Golgi secretion in plant cells.The New phytologist · 2026Article
- Arabidopsis ECHIDNA Plays an Important Role in Auxin Regulation of Clathrin-Mediated Trafficking During Root Gravitropism.Plant, cell & environment · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Clathrin-mediated trafficking regulates copper tolerance by modulating the localization of HEAVY METAL ATPase 5 in Arabidopsis root cells.Plant physiology · 2025Article
- BBSome deficiency in Lotmaria passim reveals divergent functions in trypanosomatid parasites.Parasites & vectors · 2025Article
- Constructing a Prognostic Model for Subtypes of Colorectal Cancer Based on Machine Learning and Immune Infiltration-Related Genes.Journal of cellular and molecular medicine · 2025Article
- Genome-wide identification of the adaptor protein complexes and its expression patterns analysis in foxtail millet (Setaria italica L.).BMC plant biology · 2025Article
- The role of the AP-1 adaptor complex in outgoing and incoming membrane traffic.The Journal of cell biology · 2024Article
- Endosomal protein expression of γ1-adaptin is associated with tumor growth activity and relapse-free survival in breast cancer.Breast cancer (Tokyo, Japan) · 2024Article
- Protein subcellular relocalization and function of duplicated flagellar calcium binding protein genes in honey bee trypanosomatid parasite.PLoS genetics · 2024Article
- Aspartyl protease in the secretome of honey bee trypanosomatid parasite contributes to infection of bees.Parasites & vectors · 2024Article
- An Overview of Protein Secretion in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Joint QTL Mapping and Transcriptome Sequencing Analysis Reveal Candidate Seed-Shattering-Related Genes in Common Buckwheat.International journal of molecular sciences · 2023Article
- A network of intracellular transport.Nature plants · 2023Article
- Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator.Nature plants · 2023Article
- FgAP1Journal of fungi (Basel, Switzerland) · 2023Article
20 more citing papers are in PubMed but not listed here.
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8 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Adaptor protein (AP) complexes are the predominant coat proteins of membrane vesicles in post-Golgi trafficking of mammalian cells. Each AP complex contains a specific medium subunit, μ-adaptin, that selects cargo proteins bearing sequence-specific sorting motifs. Much less is known about the AP complexes and their μ subunits in plants. Because of uncertain homology, the μ-adaptins of Arabidopsis have been designated muA through muD [Happel et al. (2004) Plant J 37(5):678-693]. Furthermore, only muD has been assigned to a specific AP complex, AP-3, involved in Golgi-vacuolar trafficking [Niihama et al. (2009) Plant Cell Physiol 50(12):2057-2068, Zwiewka et al. (2011) Cell Res 21(12):1711-1722, and Wolfenstetter et al. (2012) Plant Cell 24(1):215-232]. In contrast, the μ subunit of neither the post-Golgi trafficking AP-1 complex nor the endocytic AP-2 complex has been identified. Here, we report the functional analysis of redundant AP-1 μ-adaptins AP1M1 (also known as muB1) and AP1M2 (also known as muB2). Coimmunoprecipitation revealed that both AP1M2 and its less strongly expressed isoform AP1M1 are complexed with the large subunit γ-adaptin of AP-1. In addition, AP1M2 was localized at or near the trans-Golgi network. Knockout mutations of AP1M2 impaired pollen function and arrested plant growth whereas the ap1m1 ap1m2 double mutant was nearly pollen-lethal. At the cellular level, the absence of AP1M2 entailed inhibition of multiple trafficking pathways from the trans-Golgi network to the vacuole and to the plasma membrane in interphase and to the plane of cell division in cytokinesis. Thus, AP-1 is crucial in post-Golgi trafficking in plant cells and required for cell division and plant growth.
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