ArticleMethods in molecular biology (Clifton, N.J.)2013
Quantification of genetically controlled cell death in budding yeast.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The heat-ramp method to study regulated cell death in a pathogenic yeastbioRxiv : the preprint server for biology · 2026Article
- The PICLS high-throughput screening method for agents extending cellular longevity identifies 2,5-anhydro-D-mannitol as novel anti-aging compound.GeroScience · 2023Article
- Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization.Cell reports · 2022Article
- The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae.Biogerontology · 2022Review
- Article
- Diverse conditions support near-zero growth in yeast: Implications for the study of cell lifespan.Microbial cell (Graz, Austria) · 2019Review
- Identification of Genes Regulating Cell Death inFrontiers in microbiology · 2019Article
- Yeast as a tool to identify anti-aging compounds.FEMS yeast research · 2018Review
- Impact of Yeast Pigmentation on Heat Capture and Latitudinal Distribution.Current biology : CB · 2018Article
- Guidelines and recommendations on yeast cell death nomenclature.Microbial cell (Graz, Austria) · 2018Review
- Cell death in genome evolution.Seminars in cell & developmental biology · 2015Review
- Genome-wide consequences of deleting any single gene.Molecular cell · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Yeast are the foremost genetic model system. With relative ease, entire chemical libraries can be screened for effects on essentially every gene in the yeast genome. Until recently, researchers focused only on whether yeast were killed by the conditions applied, irrespective of the mechanisms by which they died. In contrast, considerable effort has been devoted to understanding the mechanisms of mammalian cell death. However, most of the methodologies for detecting programmed apoptotic and necrotic death of mammalian cells have not been applicable to yeast. Therefore, we developed a cell death assay for baker's yeast Saccharomyces cerevisiae to identify genes involved in the mechanisms of yeast cell death. Small volumes of yeast suspensions are subjected to a precisely controlled heat ramp, allowing sufficient time for yeast cell factors to suppress or facilitate death, which can be quantified by high-throughput automated analyses. This assay produces remarkably reliable results that typically reflect results with other death stimuli. Here we describe the protocol and its caveats, which can be easily overcome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.