ArticleChembiochem : a European journal of chemical biology2013
Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.
Article in Chembiochem : a European journal of chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Disentangling Timescales of Molecular Kinetics with spFRET using ALEX-FCS.Journal of fluorescence · 2025Article
- Photoswitchable Fluorescence of Peptide-Based Hemipiperazines Inside of Living Cells.Journal of the American Chemical Society · 2025Article
- Direct, ensemble FRET approaches to monitor transient state kinetics of human DNA polymerase δ holoenzyme assembly and initiation of DNA synthesis.Methods in enzymology · 2024Article
- A new twist on PIFE: photoisomerisation-related fluorescence enhancement.Methods and applications in fluorescence · 2023Review
- Article
- Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers.Biosensors · 2023Article
- Fluorescent labeling of plasmid DNA for gene delivery: Implications of dye hydrophobicity on labeling efficiencies and nanoparticle size.Analytical biochemistry · 2022Article
- Engineering tandem single-chain Fv as cell surface reporters with enhanced properties of fluorescence detection.Protein engineering, design & selection : PEDS · 2015Article
- Conformational transitions in the glycine-bound GluN1 NMDA receptor LBD via single-molecule FRET.Biophysical journal · 2015Article
- Structural dynamics of the glycine-binding domain of the N-methyl-D-aspartate receptor.The Journal of biological chemistry · 2015Article
- Molecular approaches to chromatography using single molecule spectroscopy.Analytical chemistry · 2015Review
- Super-resolution mbPAINT for optical localization of single-stranded DNA.ACS applied materials & interfaces · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes.
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