ArticleMolecular therapy : the journal of the American Society of Gene Therapy2013
Ultrasound-targeted microbubble destruction-mediated gene delivery into canine livers.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Submicron-sized ultrasound contrast agents as therapeutic peptide delivery vehicles in type 1 diabetes.Theranostics · 2026Article
- Transcutaneous ultrasound-mediated gene delivery into canine livers achieves therapeutic levels of factor VIII expression.Blood advances · 2022Article
- Ultrasound-mediated gene delivery of factor VIII plasmids for hemophilia A gene therapy in mice.Molecular therapy. Nucleic acids · 2022Article
- Ultrasonic particles: An approach for targeted gene delivery.Advanced drug delivery reviews · 2021Review
- Acoustically Driven Microbubbles Enable Targeted Delivery of microRNA-Loaded Nanoparticles to Spontaneous Hepatocellular Neoplasia in Canines.Advanced therapeutics · 2020Article
- Transcutaneous Ultrasound-Mediated Nonviral Gene Delivery to the Liver in a Porcine Model.Molecular therapy. Methods & clinical development · 2019Article
- Applications of Ultrasound to Stimulate Therapeutic Revascularization.International journal of molecular sciences · 2019Review
- Multifunctionalized Microscale Ultrasound Contrast Agents for Precise Theranostics of Malignant Tumors.Contrast media & molecular imaging · 2019Review
- Ultrasound-Mediated Gene Therapy in Swine Livers Using Single-Element, Multi-lensed, High-Intensity Ultrasound Transducers.Molecular therapy. Methods & clinical development · 2018Article
- Prolonging pulse duration in ultrasound-mediated gene delivery lowers acoustic pressure threshold for efficient gene transfer to cells and small animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Enhanced effect of nuclear localization signal peptide during ultrasound‑targeted microbubble destruction‑mediated gene transfection.Molecular medicine reports · 2017Article
- Ultrasound-Mediated Microbubble Destruction (UMMD) Facilitates the Delivery of CA19-9 Targeted and Paclitaxel Loaded mPEG-PLGA-PLL Nanoparticles in Pancreatic Cancer.Theranostics · 2016Article
- Therapeutic potential of ultrasound microbubbles in gastrointestinal oncology: recent advances and future prospects.Therapeutic advances in gastroenterology · 2015Review
- In Vivo Transfection and Detection of Gene Expression of Stem Cells Preloaded with DNA-carrying Microbubbles.Radiology · 2015Article
- Role of ultrasound and microbubble-mediated heat shock protein 72 siRNA on ischemia-reperfusion liver injury in rat.International journal of clinical and experimental medicine · 2015Article
- Development of therapeutic microbubbles for enhancing ultrasound-mediated gene delivery.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Ultrasound-mediated gene and drug delivery using a microbubble-liposome particle system.Theranostics · 2014Article
- One-step synthesis of platinum nanoparticles loaded in alginate bubbles.Nanoscale research letters · 2014Article
- piggyBac-mediated phenotypic correction of factor VIII deficiency.Molecular therapy. Methods & clinical development · 2014Article
- A facile fabrication of alginate microbubbles using a gas foaming reaction.Molecules (Basel, Switzerland) · 2013Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
Ultrasound (US) was applied to a targeted canine liver lobe simultaneously with injection of plasmid DNA (pDNA)/microbubble (MB) complexes into a portal vein (PV) segmental branch and occlusion of the inferior vena cava (IVC) to facilitate DNA uptake. By using a 1.1 MHz, 13 mm diameter transducer, a fivefold increase in luciferase activity was obtained at 3.3 MPa peak negative pressure (PNP) in the treated lobe. For more effective treatment of large tissue volumes in canines, a planar unfocused transducer with a large effective beam diameter (52 mm) was specifically constructed. Its apodized dual element configuration greatly reduced the near-field transaxial pressure variations, resulting in a remarkably uniform field of US exposure for the treated tissues. Together with a 15 kW capacity US amplifier, a 692-fold increase of gene expression was achieved at 2.7 MPa. Transaminase and histology analysis indicated minimal tissue damage. These experiments represent an important developmental step toward US-mediated gene delivery in large animals and clinics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.