ArticleInternational journal of molecular medicine2013
HIF-1α knockdown by miRNA decreases survivin expression and inhibits A549 cell growth in vitro and in vivo.
Article in International journal of molecular medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- C/EBPβ Regulates HIF-1α-Driven Invasion of Non-Small-Cell Lung Cancer Cells.Biomolecules · 2024Article
- Role of Hypoxia-Inducible Factors 1α (HIF1α) in SH-SY5Y Cell Autophagy Induced by Oxygen-Glucose Deprivation.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Adipocyte-activated oxidative and ER stress pathways promote tumor survival in bone via upregulation of Heme Oxygenase 1 and Survivin.Scientific reports · 2018Article
- How to Modulate Tumor Hypoxia for Preclinical In Vivo Imaging Research.Contrast media & molecular imaging · 2018Review
- HIF-2α dictates the susceptibility of pancreatic cancer cells to TRAIL by regulating survivin expression.Oncotarget · 2017Article
- TGF-β Regulates Survivin to Affect Cell Cycle and the Expression of EGFR and MMP9 in Glioblastoma.Molecular neurobiology · 2016Article
- SDF-1/CXCR4 Axis Regulates Cell Cycle Progression and Epithelial-Mesenchymal Transition via Up-regulation of Survivin in Glioblastoma.Molecular neurobiology · 2016Article
- The involvement of survivin in insulin-like growth factor 1-induced epithelial-mesenchymal transition in gastric cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- SL4, a chalcone-based compound, induces apoptosis in human cancer cells by activation of the ROS/MAPK signalling pathway.Cell proliferation · 2015Article
- The twisted survivin connection to angiogenesis.Molecular cancer · 2015Review
- Myricanol induces apoptotic cell death and anti-tumor activity in non-small cell lung carcinoma in vivo.International journal of molecular sciences · 2015Article
- SDF-1/CXCR4 signaling up-regulates survivin to regulate human sacral chondrosarcoma cell cycle and epithelial-mesenchymal transition via ERK and PI3K/AKT pathway.Medical oncology (Northwood, London, England) · 2015Article
- HIF-1α contributes to proliferation and invasiveness of neuroblastoma cells via SHH signaling.PloS one · 2015Article
- Survivin contributes to the progression of diabetic retinopathy through HIF-1α pathway.International journal of clinical and experimental pathology · 2015Article
- HIF-1α induces VE-cadherin expression and modulates vasculogenic mimicry in esophageal carcinoma cells.World journal of gastroenterology · 2014Article
- RETRACTED: Maslinic acid induces mitochondrial apoptosis and suppresses HIF-1α expression in A549 lung cancer cells under normoxic and hypoxic conditions.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study examined the downregulation of survivin expression by hypoxia-inducible factor-1α (HIF-1α) miRNA and its effect in the inhibition of A549 cell growth in vitro and in vivo. Survivin expression, apoptosis, proliferation and migration under normoxic and hypoxic conditions were assessed by standard methods. Cotransfection and chromatin immunoprecipitation were used to observe the effects of HIF-1α on survivin transcription. HIF-1α knockdown in A549 cells were injected into nude mice to examine survivin expression and suppression of tumorigenicity. Transfection of A549 cells with HIF-1α miRNA led to decreased expression of HIF-1α and survivin mRNA and protein. Survivin overexpression is mediated by HIF-1α by direct binding to a putative binding site in the survivin core promoter. HIF-1α-miRNA induced apoptosis and inhibited proliferation of A549 cells under hypoxic, but not normoxic, conditions, whereas transfection by survivin expression vectors partly rescued the apoptotic phenotype and revived cell proliferation under hypoxic conditions. However, cell migration was substantially suppressed by HIF-1α silencing under normoxic and hypoxic conditions. After A549 cells were xenografted in nude mice, survivin expression in mice treated with HIF-1α miRNA was downregulated, and tumor growth was significantly inhibited. Silenced HIF-1α gene expression induced apoptosis and suppressed growth of A549 cells by downregulating survivin expression in vitro and in vivo. Our results also provide a basis to target the HIF-1α pathway in lung cancer therapy.
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