ArticleBMC biophysics2013
Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells.
Article in BMC biophysics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Intra-cluster receptor density (IRD): A molecular switch for TNFR1 clusters' signaling.Science advances · 2026Article
- VEGFR2 dimerization kinetics on the cell surface determined by microscopy and mathematical modeling.Biophysical journal · 2026Article
- Single-Molecule FRET-Tracking of InlB-Activated MET Receptors in Living Cells.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Single-molecule imaging and molecular dynamics simulations reveal early activation of the MET receptor in cells.Nature communications · 2024Article
- Fluorescence Correlation Spectroscopy as a Versatile Method to Define Aptamer-Protein Interactions with Single-Molecule Sensitivity.Analytical chemistry · 2024Article
- Receptor tyrosine kinase MET ligand-interaction classified via machine learning from single-particle tracking data.Molecular biology of the cell · 2022Article
- A recurring packing contact in crystals of InlB pinpoints functional binding sites in the internalin domain and the B repeat.Acta crystallographica. Section D, Structural biology · 2022Article
- Article
- Quantifying the strength of heterointeractions among receptor tyrosine kinases from different subfamilies: Implications for cell signaling.The Journal of biological chemistry · 2020Article
- CX3CL1 homo-oligomerization drives cell-to-cell adherence.Scientific reports · 2020Article
- Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation.International journal of molecular sciences · 2020Article
- Correction of Systematic Bias in Single Molecule Photobleaching Measurements.Biophysical journal · 2020Article
- Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.Molecular biology of the cell · 2019Article
- Single-molecule photobleaching: Instrumentation and applications.Journal of biosciences · 2018Article
- Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking.FEBS open bio · 2017Article
- Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.eLife · 2017Article
- A New Method to Study Heterodimerization of Membrane Proteins and Its Application to Fibroblast Growth Factor Receptors.The Journal of biological chemistry · 2017Article
- MET-activating Residues in the B-repeat of the Listeria monocytogenes Invasion Protein InlB.The Journal of biological chemistry · 2016Article
- One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy.Scientific reports · 2015Article
- Activation of transmembrane cell-surface receptors via a common mechanism? The "rotation model".BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe human receptor tyrosine kinase MET and its ligand hepatocyte growth factor/scatter factor are essential during embryonic development and play an important role during cancer metastasis and tissue regeneration. In addition, it was found that MET is also relevant for infectious diseases and is the target of different bacteria, amongst them Listeria monocytogenes that induces bacterial uptake through the surface protein internalin B. Binding of ligand to the MET receptor is proposed to lead to receptor dimerization. However, it is also discussed whether preformed MET dimers exist on the cell membrane.
resultsTo address these issues we used single-molecule fluorescence microscopy techniques. Our photobleaching experiments show that MET exists in dimers on the membrane of cells in the absence of ligand and that the proportion of MET dimers increases significantly upon ligand binding.
conclusionsOur results indicate that partially preformed MET dimers may play a role in ligand binding or MET signaling. The addition of the bacterial ligand internalin B leads to an increase of MET dimers which is in agreement with the model of ligand-induced dimerization of receptor tyrosine kinases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.