ArticleIndian journal of virology : an official organ of Indian Virological Society2012
In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein.
Article in Indian journal of virology : an official organ of Indian Virological Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Cymbidium Mosaic Virus Infecting Orchids: What, How, and What Next?Iranian journal of biotechnology · 2022Article
- Heterologous expression of pepper mild mottle virus coat protein encoding region and its application in immuno-diagnostics.Virusdisease · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) with extract from CymMV-infected tissue. The results indicate that polyclonal antisera prepared from expressed CymMV coat proteins were useful for the detection of CymMV in an array of assays. The detection system developed is highly effective for detection of Indian strain of the virus in comparison to kits available in the international market.
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