ArticleEukaryotic cell2013
The plasma membrane proton pump PMA-1 is incorporated into distal parts of the hyphae independently of the Spitzenkörper in Neurospora crassa.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Fluid mechanics within mycorrhizal networks: exploring concepts, traits, and methodologies.The New phytologist · 2025Review
- Protein secretion and associated stress in industrially employed filamentous fungi.Applied microbiology and biotechnology · 2024Review
- Hyphae of the fungus Aspergillus nidulans demonstrate chemotropism to nutrients and pH.PLoS biology · 2024Article
- Golgi-Bypass Is a Major Unconventional Route for Translocation to the Plasma Membrane of Non-Apical Membrane Cargoes inFrontiers in cell and developmental biology · 2022Article
- The Exocyst Regulates Hydrolytic Enzyme Secretion at Hyphal Tips and Septa in the Banana Fusarium Wilt Fungus Fusarium odoratissimum.Applied and environmental microbiology · 2021Article
- AtfA-Independent Adaptation to the Toxic Heavy Metal Cadmium inMicroorganisms · 2021Article
- Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide.Biomolecules · 2020Article
- The critical role of plasma membrane H+-ATPase activity in cephalosporin C biosynthesis of Acremonium chrysogenum.PloS one · 2020Article
- Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion.Microbial cell factories · 2018Article
- Characterization of a Novel Prevacuolar Compartment in Neurospora crassa.Eukaryotic cell · 2015Article
- Identification of Allorecognition Loci in Neurospora crassa by Genomics and Evolutionary Approaches.Molecular biology and evolution · 2015Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most models for fungal growth have proposed a directional traffic of secretory vesicles to the hyphal apex, where they temporarily aggregate at the Spitzenkörper before they fuse with the plasma membrane (PM). The PM H(+)-translocating ATPase (PMA-1) is delivered via the classical secretory pathway (endoplasmic reticulum [ER] to Golgi) to the cell surface, where it pumps H(+) out of the cell, generating a large electrochemical gradient that supplies energy to H(+)-coupled nutrient uptake systems. To characterize the traffic and delivery of PMA-1 during hyphal elongation, we have analyzed by laser scanning confocal microscopy (LSCM) strains of Neurospora crassa expressing green fluorescent protein (GFP)-tagged versions of the protein. In conidia, PMA-1-GFP was evenly distributed at the PM. During germination and germ tube elongation, PMA-1-GFP was found all around the conidial PM and extended to the germ tube PM, but fluorescence was less intense or almost absent at the tip. Together, the data indicate that the electrochemical gradient driving apical nutrient uptake is generated from early developmental stages. In mature hyphae, PMA-1-GFP localized at the PM at distal regions (>120 μm) and in completely developed septa, but not at the tip, indicative of a distinct secretory route independent of the Spitzenkörper occurring behind the apex.
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