ArticleCytotechnology2014
Differentiation of spermatogonial stem cell-like cells from murine testicular tissue into haploid male germ cells in vitro.
Article in Cytotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- In Vitro Spermatogenesis: How Far from Clinical Application?Current urology reports · 2016Pooled it
- Apoptotic inhibitor Y-27632 improves post-thaw viability and stemness of sheep spermatogonial stem cells.Journal of assisted reproduction and genetics · 2026Article
- Fetal bovine serum in cryomedia protects sheep spermatogonial stem cells and preserves stemness characteristics during cryopreservation.In vitro cellular & developmental biology. Animal · 2025Article
- Transcriptome Analysis of Key Genes Involved in the Initiation of Spermatogonial Stem Cell Differentiation.Genes · 2024Article
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
- Roles of Spermatogonial Stem Cells in Spermatogenesis and Fertility Restoration.Frontiers in endocrinology · 2022Review
- Establishment of fishing cat cell biobanking for sustainable conservation.Frontiers in veterinary science · 2022Article
- Tissue-Nonspecific Alkaline Phosphatase, a Possible Mediator of Cell Maturation: Towards a New Paradigm.Cells · 2021Review
- Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility.Cells · 2020Review
- Development of An Artificial Male Germ Cell Niche Using Electrospun Poly Vinyl Alcohol/Human Serum Albumin/Gelatin Fibers.Cell journal · 2019Article
- Progress and future prospect ofOncotarget · 2017Review
- IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway.Scientific reports · 2016Article
- Generation of male germ cellsAsian journal of andrologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro differentiation of spermatogonial stem cells (SSCs) promotes the understanding of the mechanism of spermatogenesis. The purpose of this study was to isolate spermatogonial stem cell-like cells from murine testicular tissue, which then were induced into haploid germ cells by retinoic acid (RA). The spermatogonial stem cell-like cells were purified and enriched by a two-step plating method based on different adherence velocities of SSCs and somatic cells. Cell colonies were present after culture in M1-medium for 3 days. Through alkaline phosphatase, RT-PCR and indirect immunofluorescence cell analysis, cell colonies were shown to be SSCs. Subsequently, cell colonies of SSCs were cultured in M2-medium containing RA for 2 days. Then the cell colonies of SSCs were again cultured in M1-medium for 6-8 days, RT-PCR and indirect immunofluorescence cell analysis were chosen to detect haploid male germ cells. It could be demonstrated that 10(-7) mol l(-1) of RA effectively induced the SSCs into haploid male germ cells in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.