ArticleApplied and environmental microbiology2013
Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Review
- An agent-based model of Trypanosoma brucei social motility to explore determinants of colony pattern formation.PLoS computational biology · 2026Article
- c-di-GMP-mediated pause behavior enablesApplied and environmental microbiology · 2026Article
- Characterizing Spatiotemporal Expansion of Pseudomonas aeruginosa Communities in Polymer and Mucin Gel Environments.Biotechnology and bioengineering · 2026Article
- The type IV pilus steering committee: how Pil-Chp controls directional motility.Journal of bacteriology · 2025Review
- Article
- Bacteria can rotate while body tethered to a solid surface.Biophysical journal · 2025Article
- The type IV pilus chemoreceptor PilJ controls chemotaxis of one bacterial species towards another.PLoS biology · 2024Article
- Silver Ions Inhibit Bacterial Movement and Stall Flagellar Motor.International journal of molecular sciences · 2023Article
- Charge Mapping ofAnalytical chemistry · 2023Article
- Modeling of lophotrichous bacteria reveals key factors for swimming reorientation.Scientific reports · 2022Article
- A new mode of swimming in singly flagellatedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Flagella, Chemotaxis and Surface Sensing.Advances in experimental medicine and biology · 2022Article
- Dynamics of the Two Stator Systems in the Flagellar Motor of Pseudomonas aeruginosa Studied by a Bead Assay.Applied and environmental microbiology · 2021Article
- Vibrio cholerae Motility in Aquatic and Mucus-Mimicking Environments.Applied and environmental microbiology · 2021Article
- Review
- Dynamic swimming pattern of Pseudomonas aeruginosa near a vertical wall during initial attachment stages of biofilm formation.Scientific reports · 2021Article
- Biophysical methods to quantify bacterial behaviors at oil-water interfaces.Journal of industrial microbiology & biotechnology · 2020Review
- Article
- Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series.Journal of visualized experiments : JoVE · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of the cell's rotational trajectories. The second applies piecewise linear approximation to the accumulated rotation curve to reduce noise and separate the motion of bacteria into phases. Thus, it can separate counterclockwise (CCW) and clockwise (CW) rotations distinctly and measure rotational speed accurately. Using this program, we analyzed the properties of tethered Pseudomonas aeruginosa and Pseudomonas putida cells for the first time. We found that the Pseudomonas flagellar motor spends equal time in both CCW and CW phases and that it rotates with the same speed in both phases. In addition, we discovered that the cell body can remain stationary for short periods of time, leading to the existence of a third phase of the flagellar motor which we call "pause." In addition, P. aeruginosa cells adopt longer run lengths, fewer pause frequencies, and shorter pause durations as part of their chemotactic response. We propose that one purpose of the pause phase is to allow the cells to turn at a large angle, where we show that pause durations in free-swimming cells positively correlate with turn angle sizes. Taken together, our results suggest a new "run-reverse-turn" paradigm for polar-flagellated Pseudomonas motility that is different from the "run-and-tumble" paradigm established for peritrichous Escherichia coli.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.