ArticleApplied and environmental microbiology2013
Transcriptome sequencing (RNA-seq) analysis of the effects of metal nanoparticle exposure on the transcriptome of Chlamydomonas reinhardtii.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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Who cites it
51 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages.International journal of molecular sciences · 2020Pooled it
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- Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture.Plant molecular biology · 2024Review
- Proteomic insights to decipher nanoparticle uptake, translocation, and intercellular mechanisms in plants.Environmental science and pollution research international · 2024Review
- Palmelloid Formation and Cell Aggregation Are Essential Mechanisms for High Light Tolerance in a Natural Strain ofInternational journal of molecular sciences · 2023Article
- Metabolic alterations in algaEnvironmental science. Nano · 2022Article
- Nanoscale zero-valent iron alters physiological, biochemical, and transcriptomic response of nonylphenol-exposed algae (Dictyosphaerium sp.).Environmental science and pollution research international · 2022Article
- RNA-Seq Analysis ofFrontiers in plant science · 2022Article
- Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings.Nanomaterials (Basel, Switzerland) · 2021Article
- Analysis of Nanotoxicity with Integrated Omics and Mechanobiology.Nanomaterials (Basel, Switzerland) · 2021Review
- Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles.International journal of environmental research and public health · 2021Review
- Transcriptome Profile Alterations with Carbon Nanotubes, Quantum Dots, and Silver Nanoparticles: A Review.Genes · 2021Review
- Nanoimpact in Plants: Lessons from the Transcriptome.Plants (Basel, Switzerland) · 2021Review
- Dose-Dependent Physiological and Transcriptomic Responses of Lettuce (International journal of molecular sciences · 2021Article
- The use of HRM shifts in qPCR to investigate a much neglected aspect of interference by intracellular nanoparticles.PloS one · 2021Article
- Long-Term Toxicity of ZnO Nanoparticles onNanomaterials (Basel, Switzerland) · 2020Article
- Nanoparticles: Synthesis, Morphophysiological Effects, and Proteomic Responses of Crop Plants.International journal of molecular sciences · 2020Review
- Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor signaling in immune-competent human cell lines.Nanoscale advances · 2020Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The widespread use of nanoparticles (NPs) raises concern over their potential toxicological effects in humans and ecosystems. Here we used transcriptome sequencing (RNA-seq) to evaluate the effects of exposure to four different metal-based NPs, nano-Ag (nAg), nano-TiO2 (nTiO2), nano-ZnO (nZnO), and CdTe/CdS quantum dots (QDs), in the eukaryotic green alga Chlamydomonas reinhardtii. The transcriptome was characterized before and after exposure to each NP type. Specific toxicological effects were inferred from the functions of genes whose transcripts either increased or decreased. Data analysis resulted in important differences and also similarities among the NPs. Elevated levels of transcripts of several marker genes for stress were observed, suggesting that only nZnO caused nonspecific global stress to the cells under environmentally relevant conditions. Genes with photosynthesis-related functions were decreased drastically during exposure to nTiO2 and slightly during exposures to the other NP types. This pattern suggests either toxicological effects in the chloroplast or effects that mimic a transition from low to high light. nAg exposure dramatically elevated the levels of transcripts encoding known or predicted components of the cell wall and the flagella, suggesting that it damages structures exposed to the external milieu. Exposures to nTiO2, nZnO, and QDs elevated the levels of transcripts encoding subunits of the proteasome, suggesting proteasome inhibition, a phenomenon believed to underlie the development and progression of several major diseases, including Alzheimer's disease, and used in chemotherapy against multiple myeloma.
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