ArticleApplied and environmental microbiology2013
L-A-lus, a new variant of the L-A totivirus found in wine yeasts with Klus killer toxin-encoding Mlus double-stranded RNA: possible role of killer toxin-encoding satellite RNAs in the evolution of their helper viruses.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed.
- Article
- Genomic characterization and diversity of dsRNA viruses and their killer satellites in a natural population of the yeastVirus evolution · 2026Article
- A Comprehensive Structural and Functional Analysis ofbioRxiv : the preprint server for biology · 2025Article
- The KillerMicroorganisms · 2024Review
- RNA viruses, M satellites, chromosomal killer genes, and killer/nonkiller phenotypes in the 100-genomes S. cerevisiae strains.G3 (Bethesda, Md.) · 2023Article
- Exploring the Mycovirus Universe: Identification, Diversity, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2023Review
- The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae.FEMS yeast research · 2023Article
- Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing.Viruses · 2022Article
- Structures of L-BC virus and its open particle provide insight into Totivirus capsid assembly.Communications biology · 2022Article
- Expression of the K74 Killer Toxin from Saccharomyces paradoxus Is Modulated by the Toxin-Encoding M74 Double-Stranded RNA 5' Untranslated Terminal Region.Applied and environmental microbiology · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- A Simple Multiplex Reverse Transcription-PCR Method for the Diagnosis of L-A and M Totiviruses in Saccharomyces cerevisiae.Applied and environmental microbiology · 2022Article
- New Insights into the Genome Organization of Yeast Double-Stranded RNA LBC Viruses.Microorganisms · 2022Article
- Infection by dsRNA viruses is associated with enhanced sporulation efficiency inEcology and evolution · 2022Article
- Genome Features of a New Double-Stranded RNA Helper Virus (LBCbarr) from WineInternational journal of molecular sciences · 2021Article
- Specificity Determination inMicroorganisms · 2021Article
- Viruses of endophytic and pathogenic forest fungi.Virus genes · 2020Review
- Genome Organization of a New Double-Stranded RNA LA Helper Virus From WineFrontiers in microbiology · 2020Article
- Article
- Production of fumonisins by endophytic strains of Tolypocladium cylindrosporum and its relation to fungal virus infection.Mycotoxin research · 2018Article
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3 authors.
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Abstract
Yeast killer viruses are widely distributed in nature. Several toxins encoded in double-stranded RNA (dsRNA) satellites of the L-A totivirus have been described, including K1, K2, K28, and Klus. The 4.6-kb L-A genome encodes the Gag major structural protein that forms a 39-nm icosahedral virion and Gag-Pol, a minor fusion protein. Gag-Pol has transcriptase and replicase activities responsible for maintenance of L-A (or its satellite RNAs). Recently we reported a new killer toxin, Klus. The L-A virus in Klus strains showed poor hybridization to known L-A probes, suggesting substantial differences in their sequences. Here we report the characterization of this new L-A variant named L-A-lus. At the nucleotide level, L-A and L-A-lus showed only 73% identity, a value that increases to 86% in the amino acid composition of Gag or Gag-Pol. Two regions in their genomes, however, the frameshifting region between Gag and Pol and the encapsidation signal, are 100% identical, implying the importance of these two cis signals in the virus life cycle. L-A-lus shows higher resistance than L-A to growth at high temperature or to in vivo expression of endo- or exonucleases. L-A-lus also has wider helper activity, being able to maintain not only Mlus but also M1 or a satellite RNA of L-A called X. In a screening of 31 wine strains, we found that none of them had L-A; they carried either L-A-lus or a different L-A variant in K2 strains. Our data show that distinct M killer viruses are specifically associated with L-As with different nucleotide compositions, suggesting coevolution.
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