ArticleMicrobiology (Reading, England)2013
Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Lead contamination fixation through COScientific reports · 2026Article
- A Selective Phenyl Azo Acetate Substrate for Enhanced Carbonic Anhydrase Functional Analysis.Analytical science advances · 2026Article
- Bacterial β-carbonic anhydrases: a six-step prioritization framework for antibacterial target validation.Archives of microbiology · 2026Review
- Multi-faceted exploration of the novel active γ-carbonic anhydrase PaCAγ1 in the human pathogen Pseudomonas aeruginosa.International journal of biological macromolecules · 2025Article
- In silico analysis of hypothetical proteins in Pseudomonas aeruginosa PAC1: Structural and functional insights.Journal, genetic engineering & biotechnology · 2025Article
- Carbonic Anhydrases: A Superfamily of Ubiquitous Enzymes.International journal of molecular sciences · 2023Article
- Carbonic Anhydrase Inhibitors as Novel Antibacterials in the Era of Antibiotic Resistance: Where Are We Now?Antibiotics (Basel, Switzerland) · 2023Review
- Role of carbon-dioxide sequestering bacteria for clean air environment and prospective production of biomaterials: a sustainable approach.Environmental science and pollution research international · 2022Review
- Article
- Effect of COFrontiers in microbiology · 2019Article
- The COVeterinary research · 2018Article
- Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists.Applied and environmental microbiology · 2018Article
- Architects of nature: growing buildings with bacterial biofilms.Microbial biotechnology · 2017Article
- Cryoannealing-induced space-group transition of crystals of the carbonic anhydrase psCA3.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum.PloS one · 2016Article
- Carbon Dioxide "Trapped" in a β-Carbonic Anhydrase.Biochemistry · 2015Article
- Acid-base status is an important factor for inflammation, but don't forget CO2!Critical care (London, England) · 2014Article
- Carbon dioxide-sensing in organisms and its implications for human disease.Cellular and molecular life sciences : CMLS · 2014Review
- Preliminary X-ray crystallographic analysis of β-carbonic anhydrase psCA3 from Pseudomonas aeruginosa.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
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6 authors at 1 institution in 1 country.
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Abstract
Bacterial β-class carbonic anhydrases (CAs) are zinc metalloenzymes catalysing reversible hydration of CO2. They maintain the intracellular balance of CO2/bicarbonate required for biosynthetic reactions and represent a new group of antimicrobial drug targets. Genome sequence analysis of Pseudomonas aeruginosa PAO1, an opportunistic human pathogen causing life threatening infections, identified three genes, PAO102, PA2053 and PA4676, encoding putative β-CAs that share 28-45 % amino acid sequence identity and belong to clades A and B. The genes are conserved among all sequenced pseudomonads. The CAs were cloned, heterologously expressed and purified. Metal and enzymic analyses confirmed that the proteins contain Zn(2+) and catalyse hydration of CO2 to bicarbonate. PAO102 (psCA1) was 19-26-fold more active, and together with PA2053 (psCA2) showed CA activity at both pH 7.5 and 8.3, whereas PA4676 (psCA3) was active only at pH 8.3. Circular dichroism spectroscopy suggested that psCA2 and psCA3 undergo pH-dependent structural changes. Taken together, the data suggest that psCA1 may belong to type I and psCA3 to type II β-CAs. Immunoblot analysis showed that all three CAs are expressed in PAO1 cells when grown in ambient air and at 5 % CO2; psCA1 appeared more abundant under both conditions. Growth studies of transposon mutants showed that the disruption of psCA1 impaired PAO1 growth in ambient air and caused a minor defect at high CO2. Thus, psCA1 contributes to the adaptation of P. aeruginosa to low CO2 conditions and will be further studied for its role in virulence and as a potential antimicrobial drug target in this organism.
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