ArticleCell reports2013
Structural basis of actin filament nucleation by tandem W domains.
Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis.Communications biology · 2025Article
- Article
- Phase separation of an actin nucleator by junctional microtubules regulates epithelial function.Science advances · 2023Article
- Building the brush border, one microvillus at a time.Current opinion in cell biology · 2023Review
- Phosphorylation of the WH2 domain in yeast Las17/WASP regulates G-actin binding and protein function during endocytosis.Scientific reports · 2021Article
- Insights into Actin Polymerization and Nucleation Using a Coarse-Grained Model.Biophysical journal · 2020Article
- D-Loop Mutation G42A/G46A Decreases Actin Dynamics.Biomolecules · 2020Article
- Characterizing the actin-binding ability of Zasp52 and its contribution to myofibril assembly.PloS one · 2020Article
- Investigation into Early Steps of Actin Recognition by the Intrinsically Disordered N-WASP Domain V.International journal of molecular sciences · 2019Article
- Regulation of MRTF-A by JMY via a nucleation-independent mechanism.Cell communication and signaling : CCS · 2018Article
- Structural evidence for the roles of divalent cations in actin polymerization and activation of ATP hydrolysis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Impact of cordon-bleu expression on actin cytoskeleton architecture and dynamics.Cytoskeleton (Hoboken, N.J.) · 2016Article
- WASP family proteins, more than Arp2/3 activators.Biochemical Society transactions · 2016Review
- Cordon bleu promotes the assembly of brush border microvilli.Molecular biology of the cell · 2015Article
- Mechanisms of leiomodin 2-mediated regulation of actin filament in muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cordon Bleu serves as a platform at the basal region of microvilli, where it regulates microvillar length through its WH2 domains.Molecular biology of the cell · 2014Article
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Authors and funding
6 authors.
Funding
Abstract
Spontaneous nucleation of actin is very inefficient in cells. To overcome this barrier, cells have evolved a set of actin filament nucleators to promote rapid nucleation and polymerization in response to specific stimuli. However, the molecular mechanism of actin nucleation remains poorly understood. This is hindered largely by the fact that actin nucleus, once formed, rapidly polymerizes into filament, thus making it impossible to capture stable multisubunit actin nucleus. Here, we report an effective double-mutant strategy to stabilize actin nucleus by preventing further polymerization. Employing this strategy, we solved the crystal structure of AMPPNP-actin in complex with the first two tandem W domains of Cordon-bleu (Cobl), a potent actin filament nucleator. Further sequence comparison and functional studies suggest that the nucleation mechanism of Cobl is probably shared by the p53 cofactor JMY, but not Spire. Moreover, the double-mutant strategy opens the way for atomic mechanistic study of actin nucleation and polymerization.
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