ArticleStructure (London, England : 1993)2013
A cryptic TOG domain with a distinct architecture underlies CLASP-dependent bipolar spindle formation.
Article in Structure (London, England : 1993), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- An unconventional TOG domain is required for CLASP localization.Current biology : CB · 2023Article
- CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage.The Journal of cell biology · 2023Article
- CLASP2 facilitates dynamic actin filament organization along the microtubule lattice.Molecular biology of the cell · 2023Article
- Article
- CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state-sensitive manner.Science advances · 2023Article
- MAPping tubulin mutations.Frontiers in cell and developmental biology · 2023Review
- Article
- Dysfunction of the ciliary ARMC9/TOGARAM1 protein module causes Joubert syndrome.The Journal of clinical investigation · 2020Article
- CLASP Mediates Microtubule Repair by Restricting Lattice Damage and Regulating Tubulin Incorporation.Current biology : CB · 2020Article
- CLASPs at a glance.Journal of cell science · 2020Review
- CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments.The Journal of cell biology · 2020Article
- Article
- CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain.Developmental cell · 2018Article
- Microtubule dynamics: an interplay of biochemistry and mechanics.Nature reviews. Molecular cell biology · 2018Review
- An isolated CLASP TOG domain suppresses microtubule catastrophe and promotes rescue.Molecular biology of the cell · 2018Article
- Human CLASP2 specifically regulates microtubule catastrophe and rescue.Molecular biology of the cell · 2018Article
- TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.The Journal of cell biology · 2017Article
- Five factors can reconstitute all three phases of microtubule polymerization dynamics.The Journal of cell biology · 2016Article
- Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?Seminars in cell & developmental biology · 2016Review
- Biophysical and Structural Characterization of the Centriolar Protein Cep104 Interaction Network.The Journal of biological chemistry · 2016Article
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Abstract
CLASP is a key regulator of microtubule (MT) dynamics and bipolar mitotic spindle structure with CLASP mutants displaying a distinctive monopolar spindle phenotype. It has been postulated that cryptic TOG domains underlie CLASP’s ability to regulate MT dynamics. Here, we report the crystal structure of a cryptic TOG domain (TOG2) from human CLASP1, demonstrating the presence of a TOG array in the CLASP family. Strikingly, CLASP1 TOG2 exhibits a convex architecture across the tubulin-binding surface that contrasts with the flat tubulin-binding surface of XMAP215 family TOG domains. Mutations in key conserved TOG2 determinants abrogate the ability of CLASP mutants to rescue bipolar spindle formation in Drosophila cells depleted of endogenous CLASP. These findings highlight the common mechanistic use of TOG domains in XMAP215 and CLASP families to regulate MT dynamics and suggest that differential TOG domain architecture may confer distinct functions to these critical cytoskeletal regulators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.