ArticleBMC genomics2013
Actinobacillus pleuropneumoniae genes expression in biofilms cultured under static conditions and in a drip-flow apparatus.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 44 citations in OpenAlex.
- The morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm.Veterinary research · 2023Article
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- Recombinant of the Staphylococcal Bacteriophage Lysin CHAPMicroorganisms · 2020Article
- Relation between Biofilm and Virulence in Vibrio tapetis: A Transcriptomic Study.Pathogens (Basel, Switzerland) · 2018Article
- The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence inFrontiers in cellular and infection microbiology · 2018Article
- Incorporation ofFrontiers in veterinary science · 2018Article
- QseC Mediates Osmotic Stress Resistance and Biofilm Formation inFrontiers in microbiology · 2018Article
- Actinobacillus pleuropneumoniae grows as aggregates in the lung of pigs: is it time to refine our in vitro biofilm assays?Microbial biotechnology · 2017Article
- Mannheimia haemolytica biofilm formation on bovine respiratory epithelial cells.Veterinary microbiology · 2016Article
- A TolC-Like Protein ofFrontiers in microbiology · 2016Article
- Surface Polysaccharide Mutants Reveal that Absence of O Antigen Reduces Biofilm Formation of Actinobacillus pleuropneumoniae.Infection and immunity · 2016Article
- High-throughput microfluidic method to study biofilm formation and host-pathogen interactions in pathogenic Escherichia coli.Applied and environmental microbiology · 2015Article
- Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis.BMC research notes · 2015Article
- Development of a Laboratory Model of a Phototroph-Heterotroph Mixed-Species Biofilm at the Stone/Air Interface.Frontiers in microbiology · 2015Article
- Biofilm formation by virulent and non-virulent strains of Haemophilus parasuis.Veterinary research · 2014Article
- Method to grow Actinobacillus pleuropneumoniae biofilm on a biotic surface.BMC veterinary research · 2013Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundActinobacillus pleuropneumoniae is the Gram-negative bacterium responsible for porcine pleuropneumonia. This respiratory infection is highly contagious and characterized by high morbidity and mortality. The objectives of our study were to study the transcriptome of A. pleuropneumoniae biofilms at different stages and to develop a protocol to grow an A. pleuropneumoniae biofilm in a drip-flow apparatus. This biofilm reactor is a system with an air-liquid interface modeling lung-like environment. Bacteria attached to a surface (biofilm) and free floating bacteria (plankton) were harvested for RNA isolation. Labelled cDNA was hybridized to a microarray to compare the expression profiles of planktonic cells and biofilm cells.
resultsIt was observed that 47 genes were differentially expressed (22 up, 25 down) in a 4 h-static growing/maturing biofilm and 117 genes were differentially expressed (49 up, 68 down) in a 6h-static dispersing biofilm. The transcriptomes of a 4 h biofilm and a 6 h biofilm were also compared and 456 genes (235 up, 221 down) were identified as differently expressed. Among the genes identified in the 4 h vs 6h biofilm experiment, several regulators of stress response were down-regulated and energy metabolism associated genes were up-regulated. Biofilm bacteria cultured using the drip-flow apparatus differentially expressed 161 genes (68 up, 93 down) compared to the effluent bacteria. Cross-referencing of differentially transcribed genes in the different assays revealed that drip-flow biofilms shared few differentially expressed genes with static biofilms (4 h or 6 h) but shared several differentially expressed genes with natural or experimental infections in pigs.
conclusionThe formation of a static biofilm by A. pleuropneumoniae strain S4074 is a rapid process and transcriptional analysis indicated that dispersal observed at 6 h is driven by nutritional stresses. Furthermore, A. pleuropneumoniae can form a biofilm under low-shear force in a drip-flow apparatus and analyses indicated that the formation of a biofilm under low-shear force requires a different sub-set of genes than a biofilm grown under static conditions. The drip-flow apparatus may represent the better in vitro model to investigate biofilm formation of A. pleuropneumoniae.
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