ArticleThe Plant journal : for cell and molecular biology2013
Using a periclinal chimera to unravel layer-specific gene expression in plants.
Article in The Plant journal : for cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- The petal identity geneeLife · 2026Article
- Genome degradation in plant tissue culture.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Insights into glandular trichome biology from analysis of organ-specific gene expression programmes in cannabis, hop and tomato.The Plant journal : for cell and molecular biology · 2026Article
- A Study on the Cell Layer Patterns of a Citrus Periclinal Chimera Reveals β-Cryptoxanthin Regulation in Citrus Fruits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Modern and historical uses of plant grafting to engineer development, stress tolerance, chimeras, and hybrids.The Plant journal : for cell and molecular biology · 2025Review
- Quantitative Analysis of Tomato Flower Pedicel Abscission and Single-Cell Transcriptome Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Incomplete genome doubling enables to consistently enhance plant growth for maximum biomass production by altering multiple transcript co-expression networks in potato.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Unraveling Cuticle Formation, Structure, and Properties by Using Tomato Genetic Diversity.Frontiers in plant science · 2021Review
- Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in TwoPlants (Basel, Switzerland) · 2020Article
- The Tomato MIXTA-Like Transcription Factor Coordinates Fruit Epidermis Conical Cell Development and Cuticular Lipid Biosynthesis and Assembly.Plant physiology · 2015Article
- A small indel mutation in an anthocyanin transporter causes variegated colouration of peach flowers.Journal of experimental botany · 2015Article
- The genome of the stress-tolerant wild tomato species Solanum pennellii.Nature genetics · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Plant organs are made from multiple cell types, and defining the expression level of a gene in any one cell or group of cells from a complex mixture is difficult. Dicotyledonous plants normally have three distinct layers of cells, L1, L2 and L3. Layer L1 is the single layer of cells making up the epidermis, layer L2 the single cell sub-epidermal layer and layer L3 constitutes the rest of the internal cells. Here we show how it is possible to harvest an organ and characterise the level of layer-specific expression by using a periclinal chimera that has its L1 layer from Solanum pennellii and its L2 and L3 layers from Solanum lycopersicum. This is possible by measuring the level of the frequency of species-specific transcripts. RNA-seq analysis enabled the genome-wide assessment of whether a gene is expressed in the L1 or L2/L3 layers. From 13 277 genes that are expressed in both the chimera and the parental lines and with at least one polymorphism between the parental alleles, we identified 382 genes that are preferentially expressed in L1 in contrast to 1159 genes in L2/L3. Gene ontology analysis shows that many genes preferentially expressed in L1 are involved in cutin and wax biosynthesis, whereas numerous genes that are preferentially expressed in L2/L3 tissue are associated with chloroplastic processes. These data indicate the use of such chimeras and provide detailed information on the level of layer-specific expression of genes.
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