ArticleBMC biotechnology2013
Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine.
Article in BMC biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Unlocking the Bioactive Potential of Streptomyces: A Multidisciplinary Approach to Strain Improvement.MicrobiologyOpen · 2026Review
- Redox engineering of thermophilic fungusMycology · 2026Article
- Improvement in L-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum.Microbial cell factories · 2021Article
- Metabolic engineering strategy for synthetizing trans-4-hydroxy-L-proline in microorganisms.Microbial cell factories · 2021Review
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms.Journal of microbiology and biotechnology · 2020Review
- A Supramolecular Approach to Structure-Based Design with A Focus on Synthons Hierarchy in Ornithine-Derived Ligands: Review, Synthesis, Experimental and in Silico Studies.Molecules (Basel, Switzerland) · 2020Review
- Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in L-ornithine production.Microbial cell factories · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Determination of growth-coupling strategies and their underlying principles.BMC bioinformatics · 2019Article
- The Plasma NADRejuvenation research · 2019Article
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
- Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway.Microbial cell factories · 2018Article
- Improved L-ornithine production in Corynebacterium crenatum by introducing an artificial linear transacetylation pathway.Journal of industrial microbiology & biotechnology · 2018Article
- Improvement of L-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114.AMB Express · 2018Article
- Metabolic evolution and a comparative omics analysis of Corynebacterium glutamicum for putrescine production.Journal of industrial microbiology & biotechnology · 2018Article
- NAD Kinases: Metabolic Targets Controlling Redox Co-enzymes and Reducing Power Partitioning in Plant Stress and Development.Frontiers in plant science · 2018Review
- Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.Microbial cell factories · 2017Article
- Transcriptomic Changes in Response to Putrescine Production in Metabolically EngineeredFrontiers in microbiology · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundL-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achieve improved cellular properties without requiring metabolic or regulatory details of the strain. The evolved strains can be further optimised by metabolic engineering. Thus, metabolic evolution strategy was used for engineering Corynebacterium glutamicum to enhance L-ornithine production.
resultsA C. glutamicum strain was engineered by using a combination of gene deletions and adaptive evolution with 70 passages of growth-based selection. The metabolically evolved C. glutamicum strain, named ΔAPE6937R42, produced 24.1 g/L of L-ornithine in a 5-L bioreactor. The mechanism used by C. glutamicum ΔAPE6937R42 to produce L-ornithine was investigated by analysing transcriptional levels of select genes and NADPH contents. The upregulation of the transcription levels of genes involved in the upstream pathway of glutamate biosynthesis and the elevated NADPH concentration caused by the upregulation of the transcriptional level of the ppnK gene promoted L-ornithine production in C. glutamicum ΔAPE6937R42.
conclusionsThe availability of NADPH plays an important role in L-ornithine production in C. glutamicum. Our results demonstrated that the combination of growth-coupled evolution with analysis of transcript abundances provides a strategy to engineer microbial strains for improving production of target compounds.
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