ArticleBMC genomics2013
Microarray and deep sequencing cross-platform analysis of the mirRNome and isomiR variation in response to epidermal growth factor.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Stumbling on elusive cargo: how isomiRs challenge microRNA detection and quantification, the case of extracellular vesicles.Journal of extracellular vesicles · 2020Article
- Conserved temporal ordering of promoter activation implicates common mechanisms governing the immediate early response across cell types and stimuli.Open biology · 2018Article
- Bioengineered Noncoding RNAs Selectively Change Cellular miRNome Profiles for Cancer Therapy.The Journal of pharmacology and experimental therapeutics · 2018Article
- Profiling and Bioinformatic Analyses Indicate Differential circRNA and miRNA/isomiR Expression and Interactions.BioMed research international · 2018Article
- Characterizing isomiR variants within the microRNA-34/449 family.FEBS letters · 2017Article
- IsomiR expression patterns in canonical and Dicer‑independent microRNAs.Molecular medicine reports · 2017Article
- Reduced miR-550a-3p leads to breast cancer initiation, growth, and metastasis by increasing levels of ERK1 and 2.Oncotarget · 2016Article
- Synteny and comparative analysis of miRNA retention, conservation, and structure across Brassicaceae reveals lineage- and sub-genome-specific changes.Functional & integrative genomics · 2016Article
- miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels.Scientific reports · 2016Article
- Dengue virus infection alters post-transcriptional modification of microRNAs in the mosquito vector Aedes aegypti.Scientific reports · 2015Article
- MicroRNAs and Growth Factors: An Alliance Propelling Tumor Progression.Journal of clinical medicine · 2015Review
- Dual regulation by microRNA-200b-3p and microRNA-200b-5p in the inhibition of epithelial-to-mesenchymal transition in triple-negative breast cancer.Oncotarget · 2015Article
- Transcriptional dynamics reveal critical roles for non-coding RNAs in the immediate-early response.PLoS computational biology · 2015Article
- bacterial infection drives the expression dynamics of microRNAs and their isomiRs.PLoS genetics · 2015Article
- Identification of tubular injury microRNA biomarkers in urine: comparison of next-generation sequencing and qPCR-based profiling platforms.BMC genomics · 2014Article
- Gene expression resulting from PrPC ablation and PrPC overexpression in murine and cellular models.Molecular neurobiology · 2014Review
- Comparative microRNA expression profiles of cynomolgus monkeys, rat, and human reveal that mir-182 is involved in T2D pathogenic processes.Journal of diabetes research · 2014Article
- Regulation of miRNA processing and miRNA mediated gene repression in cancer.MicroRNA (Shariqah, United Arab Emirates) · 2014Article
- Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila.Genes & development · 2014Article
Corrections and comments
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Authors and funding
18 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEpidermal Growth Factor (EGF) plays an important function in the regulation of cell growth, proliferation, and differentiation by binding to its receptor (EGFR) and providing cancer cells with increased survival responsiveness. Signal transduction carried out by EGF has been extensively studied at both transcriptional and post-transcriptional levels. Little is known about the involvement of microRNAs (miRNAs) in the EGF signaling pathway. miRNAs have emerged as major players in the complex networks of gene regulation, and cancer miRNA expression studies have evidenced a direct involvement of miRNAs in cancer progression.
resultsIn this study, we have used an integrative high content analysis approach to identify the specific miRNAs implicated in EGF signaling in HeLa cells as potential mediators of cancer mediated functions. We have used microarray and deep-sequencing technologies in order to obtain a global view of the EGF miRNA transcriptome with a robust experimental cross-validation. By applying a procedure based on Rankprod tests, we have delimited a solid set of EGF-regulated miRNAs. After validating regulated miRNAs by reverse transcription quantitative PCR, we have derived protein networks and biological functions from the predicted targets of the regulated miRNAs to gain insight into the potential role of miRNAs in EGF-treated cells. In addition, we have analyzed sequence heterogeneity due to editing relative to the reference sequence (isomiRs) among regulated miRNAs.
conclusionsWe propose that the use of global genomic miRNA cross-validation derived from high throughput technologies can be used to generate more reliable datasets inferring more robust networks of co-regulated predicted miRNA target genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.