ArticleMolecular plant pathology2013
A two-component histidine kinase Shk1 controls stress response, sclerotial formation and fungicide resistance in Sclerotinia sclerotiorum.
Article in Molecular plant pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 99 citations in OpenAlex.
- Induced Expression ofMicroorganisms · 2023Article
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- Genetic breakthroughs in theFrontiers in plant science · 2023Review
- Preliminary Study of Resistance Mechanism ofMolecules (Basel, Switzerland) · 2022Article
- Wide distribution of resistance to the fungicides fludioxonil and iprodione in Penicillium species.PloS one · 2022Article
- Host-Induced Gene Silencing of a G Protein α Subunit GeneJournal of fungi (Basel, Switzerland) · 2021Article
- Regulation of biotic interactions and responses to abiotic stresses by MAP kinase pathways in plant pathogenic fungi.Stress biology · 2021Review
- Analysis of differentially expressed Sclerotinia sclerotiorum genes during the interaction with moderately resistant and highly susceptible chickpea lines.BMC genomics · 2021Article
- The Notorious Soilborne Pathogenic FungusPathogens (Basel, Switzerland) · 2019Review
- Overexpression of the CORVET complex alleviates the fungicidal effects of fludioxonil on the yeastThe Journal of biological chemistry · 2019Article
- The Autophagy GeneApplied and environmental microbiology · 2018Article
- The septins FaCdc3 and FaCdc12 are required for cytokinesis and affect asexual and sexual development, lipid metabolism and virulence in Fusarium asiaticum.Molecular plant pathology · 2017Article
- An atypical forkhead-containing transcription factor SsFKH1 is involved in sclerotial formation and is essential for pathogenicity in Sclerotinia sclerotiorum.Molecular plant pathology · 2017Article
- Involvement of BcElp4 in vegetative development, various environmental stress response and virulence of Botrytis cinerea.Microbial biotechnology · 2017Article
- Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus.BMC genomics · 2017Article
- New insights from an old mutant: SPADIX4 governs fruiting body development but not hyphal fusion in Sordaria macrospora.Molecular genetics and genomics : MGG · 2017Article
- Spontaneous and CRISPR/Cas9-induced mutation of the osmosensor histidine kinase of the canola pathogenFungal biology and biotechnology · 2017Article
- BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea.Scientific reports · 2016Article
- Dissecting the role of histidine kinase and HOG1 mitogen-activated protein kinase signalling in stress tolerance and pathogenicity of Parastagonospora nodorum on wheat.Microbiology (Reading, England) · 2016Article
- The eukaryotic protein kinase superfamily of the necrotrophic fungal plant pathogen, Sclerotinia sclerotiorum.Molecular plant pathology · 2016Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal histidine kinases (HKs) are involved in osmotic and oxidative stress responses, hyphal development, fungicide sensitivity and virulence. Members of HK class III are known to signal through the high-osmolarity glycerol mitogen-activated protein kinase (HOG MAPK). In this study, we characterized the Shk1 gene (SS1G_12694.3), which encodes a putative class III HK, from the plant pathogen Sclerotinia sclerotiorum. Disruption of Shk1 resulted in resistance to phenylpyrrole and dicarboximide fungicides and increased sensitivity to hyperosmotic stress and H2 O2 -induced oxidative stress. The Shk1 mutant showed a significant reduction in vegetative hyphal growth and was unable to produce sclerotia. Quantitative real-time polymerase chain reaction (qRT-PCR and glycerol determination assays showed that the expression of SsHOG1 (the last kinase of the Hog pathway) and glycerol accumulation were regulated by the Shk1 gene, but PAK (p21-activated kinase) was not. In addition, the Shk1 mutant showed no change in virulence. All the defects were restored by genetic complementation of the Shk1 deletion mutant with the wild-type Shk1 gene. These findings indicate that Shk1 is involved in vegetative differentiation, sclerotial formation, glycerol accumulation and adaption to hyperosmotic and oxidative stresses, and to fungicides, in S. sclerotiorum. Taken together, our results demonstrate, for the first time, the role of two-component HKs in Sclerotinia.
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