ArticlePloS one2013
Functional analyse of GLUT1 and GLUT12 in glucose uptake in goat mammary gland epithelial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Productive and physiological implications of top-dress addition of branched-chain amino acids and arginine on lactating sows and offspring.Journal of animal science and biotechnology · 2023Article
- Effects of a High-Grain Diet With a Buffering Agent on Milk Protein Synthesis in Lactating Goats.Frontiers in veterinary science · 2021Article
- Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.BMC veterinary research · 2016Article
- 1,25-Dihydroxyvitamin D3 modulates calcium transport in goat mammary epithelial cells in a dose- and energy-dependent manner.Journal of animal science and biotechnology · 2016Article
- Association between DNA Methylation in Whole Blood and Measures of Glucose Metabolism: KORA F4 Study.PloS one · 2016Article
- Phylogenesis and Biological Characterization of a New Glucose Transporter in the Chicken (Gallus gallus), GLUT12.PloS one · 2015Article
- The functional and molecular entities underlying amino acid and peptide transport by the mammary gland under different physiological and pathological conditions.Journal of mammary gland biology and neoplasia · 2014Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.
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