ArticlePloS one2013
Alternative sigma factor over-expression enables heterologous expression of a type II polyketide biosynthetic pathway in Escherichia coli.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Development of a High Anthraquinone-ProducingACS synthetic biology · 2025Article
- Strategic Acyl Carrier Protein Engineering Enables Functional Type II Polyketide Synthase Reconstitution In Vitro.ACS chemical biology · 2025Article
- Hierarchical metabolic engineering for rewiring cellular metabolism.FEMS microbiology reviews · 2025Review
- Investigating and Optimizing the Lysate-Based Expression of Nonribosomal Peptide Synthetases Using a Reporter System.ACS synthetic biology · 2023Article
- Metabolic Engineering and Synthetic Biology Approaches for the Heterologous Production of Aromatic Polyketides.International journal of molecular sciences · 2023Review
- High-Titer Production of the Fungal Anhydrotetracycline, TAN-1612, in Engineered Yeasts.ACS synthetic biology · 2022Article
- The σScientific reports · 2021Article
- Biosynthesis of aromatic polyketides in microorganisms using type II polyketide synthases.Microbial cell factories · 2020Review
- A chromatogram-simplified Streptomyces albus host for heterologous production of natural products.Antonie van Leeuwenhoek · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli.PLoS biology · 2019Article
- Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts.MedChemComm · 2019Review
- Activation of microbial secondary metabolic pathways: Avenues and challenges.Synthetic and systems biotechnology · 2018Review
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- Article
- Overexpression of the primary sigma factor geneMetabolic engineering communications · 2017Article
- Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes.Annals of the New York Academy of Sciences · 2017Review
- Systems Metabolic Engineering of Escherichia coli.EcoSal Plus · 2016Review
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHeterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways. Development of an effective, general heterologous expression system that can be applied to bioprospecting from metagenomic DNA will enable the discovery of a wealth of new natural products. METHODOLOGY: We have developed a new Escherichia coli-based heterologous expression system for polyketide biosynthetic gene clusters. We have demonstrated the over-expression of the alternative sigma factor σ(54) directly and positively regulates heterologous expression of the oxytetracycline biosynthetic gene cluster in E. coli. Bioinformatics analysis indicates that σ(54) promoters are present in nearly 70% of polyketide and non-ribosomal peptide biosynthetic pathways.
conclusionsWe have demonstrated a new mechanism for heterologous expression of the oxytetracycline polyketide biosynthetic pathway, where high-level pleiotropic sigma factors from the heterologous host directly and positively regulate transcription of the non-native biosynthetic gene cluster. Our bioinformatics analysis is consistent with the hypothesis that heterologous expression mediated by the alternative sigma factor σ(54) may be a viable method for the production of additional polyketide products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.