ArticlePloS one2013
Microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 123 citations in OpenAlex.
- High-energy-density acoustofluidic device using a double-parabolic ultrasonic transducer.Physical review applied · 2025Article
- Two-Step Acoustic Cell Separation Based on Cell Size and Acoustic Impedance─toward Isolation of Viable Circulating Tumor Cells.Analytical chemistry · 2025Article
- Robot-assisted chirality-tunable acoustic vortex tweezers for contactless, multifunctional, 4-DOF object manipulation.Science advances · 2024Article
- Acoustic Enrichment of Heterogeneous Circulating Tumor Cells and Clusters from Metastatic Prostate Cancer Patients.Analytical chemistry · 2024Article
- Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing.Scientific reports · 2024Article
- Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer.medRxiv : the preprint server for health sciences · 2023Article
- Fully Microfabricated Surface Acoustic Wave Tweezer for Collection of Submicron Particles and Human Blood Cells.ACS applied materials & interfaces · 2023Article
- Microfluidic techniques for isolation, formation, and characterization of circulating tumor cells and clusters.APL bioengineering · 2022Review
- Rosette-induced separation of T cells by acoustophoresis.Biomicrofluidics · 2022Article
- Recent advances in acoustic microfluidics and its exemplary applications.Biomicrofluidics · 2022Review
- Two-Step Acoustophoresis Separation of Live Tumor Cells from Whole Blood.Analytical chemistry · 2021Article
- Label-free separation of neuroblastoma patient-derived xenograft (PDX) cells from hematopoietic progenitor cell products by acoustophoresis.Stem cell research & therapy · 2021Article
- Ultrasonic Particle Manipulation in Glass Capillaries: A Concise Review.Micromachines · 2021Review
- Antibody Conjugate Assembly on Ultrasound-Confined Microcarrier Particles.ACS biomaterials science & engineering · 2020Article
- Spatially selective manipulation of cells with single-beam acoustical tweezers.Nature communications · 2020Article
- Microfluidic single-cell analysis-Toward integration and total on-chip analysis.Biomicrofluidics · 2020Review
- Differential impedance spectra analysis reveals optimal actuation frequency in bulk mode acoustophoresis.Scientific reports · 2019Article
- Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Hybrid microfluidic sorting of rare cells based on high throughput inertial focusing and high accuracy acoustic manipulation.RSC advances · 2019Article
- Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis - towards cell processing of complex biological samples.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 5 countries.
Funding
Abstract
backgroundThe use of acoustic forces to manipulate particles or cells at the microfluidic scale (i.e. acoustophoresis), enables non-contact, label-free separation based on intrinsic cell properties such as size, density and compressibility. Acoustophoresis holds great promise as a cell separation technique in several research and clinical areas. However, it has been suggested that the force acting upon cells undergoing acoustophoresis may impact cell viability, proliferation or cell function via subtle phenotypic changes. If this were the case, it would suggest that the acoustophoresis method would be a less useful tool for many cell analysis applications as well as for cell therapy.
methodsWe investigate, for the first time, several key aspects of cellular changes following acoustophoretic processing. We used two settings of ultrasonic actuation, one that is used for cell sorting (10 Vpp operating voltage) and one that is close to the maximum of what the system can generate (20 Vpp). We used microglial cells and assessed cell viability and proliferation, as well as the inflammatory response that is indicative of more subtle changes in cellular phenotype. Furthermore, we adapted a similar methodology to monitor the response of human prostate cancer cells to acoustophoretic processing. Lastly, we analyzed the respiratory properties of human leukocytes and thrombocytes to explore if acoustophoretic processing has adverse effects.
resultsBV2 microglia were unaltered after acoustophoretic processing as measured by apoptosis and cell turnover assays as well as inflammatory cytokine response up to 48 h following acoustophoresis. Similarly, we found that acoustophoretic processing neither affected the cell viability of prostate cancer cells nor altered their prostate-specific antigen secretion following androgen receptor activation. Finally, human thrombocytes and leukocytes displayed unaltered mitochondrial respiratory function and integrity after acoustophoretic processing.
conclusionWe conclude that microchannel acoustophoresis can be used for effective continuous flow-based cell separation without affecting cell viability, proliferation, mitochondrial respiration or inflammatory status.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.