ArticlePloS one2013
Ethanol diverts early neuronal differentiation trajectory of embryonic stem cells by disrupting the balance of lineage specifiers.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Integrated Network and Transcriptomic Analyses Identify a Candidate Ethanol-ADAM17 Molecular Axis Associated with Glioblastoma Progression.International journal of molecular sciences · 2026Article
- The MDPV Derivative α-PHP Regulates Cellular Differentiation and Triggers Apoptotic Cell Death and Ultrastructural Changes in Murine 3D Neurospheres.Molecules (Basel, Switzerland) · 2026Article
- Partner Smoking/Drinking Is Associated with Increased Risk of Gestational Diabetes Mellitus and Perinatal Outcomes.International journal of behavioral medicine · 2026Article
- Effects of alcohol on the transcriptome, methylome and metabolome of in vitro gastrulating human embryonic cells.Disease models & mechanisms · 2025Article
- Graft-Host Interaction and Its Effect on Wound Repair Using Mouse Models.International journal of molecular sciences · 2023Article
- Impact of alcohol exposure on neural development and network formation in human cortical organoids.Molecular psychiatry · 2023Article
- Chromatin modifier developmental pluripotency associated factor 4 (DPPA4) is a candidate gene for alcohol-induced developmental disorders.BMC medicine · 2022Article
- Alcohol's Impact on the Fetus.Nutrients · 2021Review
- Review
- Modification of stem cell states by alcohol and acetaldehyde.Chemico-biological interactions · 2020Review
- Different Effects of Knockouts in ALDH2 and ACSS2 on Embryonic Stem Cell Differentiation.Alcoholism, clinical and experimental research · 2019Article
- Ethanol promotes differentiation of embryonic stem cells through retinoic acid receptor-γ.The Journal of biological chemistry · 2019Article
- Genome-Wide Transcriptome Landscape of Embryonic Brain-Derived Neural Stem Cells Exposed to Alcohol with Strain-Specific Cross-Examination in BL6 and CD1 Mice.Scientific reports · 2019Article
- Stem cells under the influence of alcohol: effects of ethanol consumption on stem/progenitor cells.Cellular and molecular life sciences : CMLS · 2019Review
- Atypical fetal development: Fetal alcohol syndrome, nutritional deprivation, teratogens, and risk for neurodevelopmental disorders and psychopathology.Development and psychopathology · 2018Review
- Gestational Alcohol Exposure Altered DNA Methylation Status in the Developing Fetus.International journal of molecular sciences · 2017Review
- Maternal Factors that Induce Epigenetic Changes Contribute to Neurological Disorders in Offspring.Genes · 2017Review
- Review
- Prenatal Alcohol Exposure Affects Progenitor Cell Numbers in Olfactory Bulbs and Dentate Gyrus of Vervet Monkeys.Brain sciences · 2016Article
- A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration.BMC genomics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEthanol is a toxin responsible for the neurodevelopmental deficits of Fetal Alcohol Spectrum Disorders (FASD). Recent evidence suggests that ethanol modulates the protein expression of lineage specifier transcription factors Oct4 (Pou5f1) and Sox2 in early stages of mouse embryonic stem (ES) cell differentiation. We hypothesized that ethanol induced an imbalance in the expression of Oct4 and Sox2 in early differentiation, that dysregulated the expression of associated and target genes and signaling molecules and diverted cells from neuroectodermal (NE) formation. METHODOLOGY/PRINCIPAL
findingsWe showed modulation by ethanol of 33 genes during ES cell differentiation, using high throughput microfluidic dynamic array chips measuring 2,304 real time quantitative PCR assays. Based on the overall gene expression dynamics, ethanol drove cells along a differentiation trajectory away from NE fate. These ethanol-induced gene expression changes were observed as early as within 2 days of differentiation, and were independent of cell proliferation or apoptosis. Gene expression changes were correlated with fewer βIII-tubulin positive cells of an immature neural progenitor phenotype, as well as a disrupted actin cytoskeleton were observed. Moreover, Tuba1a and Gapdh housekeeping genes were modulated by ethanol during differentiation and were replaced by a set of ribosomal genes with stable expression. CONCLUSIONS/SIGNIFICANCE: These findings provided an ethanol-response gene signature and pointed to the transcriptional dynamics underlying lineage imbalance that may be relevant to FASD phenotype.
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