ArticlePloS one2013
An extensive targeted proteomic analysis of disease-related protein biomarkers in urine from healthy donors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants.Scientific reports · 2020Trial
- Kidney Injury Urine Biomarker Normal Ranges in Children.Kidney international reports · 2026Article
- Diagnostic role of urine human epididymis protein 4 in ovarian cancer.Biochemia medica · 2024Review
- Matched serum- and urine-derived biomarkers of interstitial cystitis/bladder pain syndrome.PloS one · 2024Article
- An Improved Prediction Model for Ovarian Cancer Using Urinary Biomarkers and a Novel Validation Strategy.International journal of molecular sciences · 2019Article
- Establishing a many-cytokine signature via multivariate anomaly detection.Scientific reports · 2019Article
- Comprehensive Analysis of Individual Variation in the Urinary Proteome Revealed Significant Gender Differences.Molecular & cellular proteomics : MCP · 2019Article
- Fast quantitative urinary proteomic profiling workflow for biomarker discovery in kidney cancer.Clinical proteomics · 2018Article
- A comprehensive analysis and annotation of human normal urinary proteome.Scientific reports · 2017Article
- Current state of the art for enhancing urine biomarker discovery.Expert review of proteomics · 2016Review
- Urinary protein biomarkers in the early detection of lung cancer.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Platelets, a reliable source for peripheral Alzheimer's disease biomarkers?Acta neuropathologica communications · 2014Review
- Beta2-microglobulin as a diagnostic marker in cerebrospinal fluid: a follow-up study.Disease markers · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The analysis of protein biomarkers in urine is expected to lead to advances in a variety of clinical settings. Several characteristics of urine including a low-protein matrix, ease of testing and a demonstrated proteomic stability offer distinct advantages over current widely used biofluids, serum and plasma. Improvements in our understanding of the urine proteome and in methods used in its evaluation will facilitate the clinical development of urinary protein biomarkers. Multiplexed bead-based immunoassays were utilized to evaluate 211 proteins in urines from 103 healthy donors. An additional 25 healthy donors provided serial urine samples over the course of two days in order to assess temporal variation in selected biomarkers. Nearly one-third of the evaluated biomarkers were detected in urine at levels greater than 1 ng/ml, representing a diverse panel of proteins with respect to structure, function and biological role. The presence of several biomarkers in urine was confirmed by western blot. Several methods of data normalization were employed to assess impact on biomarker variability. A complex pattern of correlations with urine creatinine, albumin and beta-2-microglobulin was observed indicating the presence of highly specific mechanisms of renal filtration. Further investigation of the urinary protein biomarkers identified in this preliminary study along with a consideration of the underlying proteomic trends suggested by these findings should lead to an improved capability to identify candidate biomarkers for clinical development.
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Registered trials
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