ArticleThe Plant cell2013
Cytochrome P450 CYP89A9 is involved in the formation of major chlorophyll catabolites during leaf senescence in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 140 citations in OpenAlex.
- Meta-QTL analysis and in-silico transcriptome assessment for controlling chlorophyll traits in common wheat.The plant genome · 2023Pooled it
- Phyllobilins: Emerging Bioactive Chlorophyll Metabolites and Their Potential Impact on Human Health.Antioxidants (Basel, Switzerland) · 2026Review
- Unexpected Dual Function of Plant YUCCA Enzymes Links Chlorophyll Catabolism to Auxin Homeostasis.Angewandte Chemie (International ed. in English) · 2026Article
- Multimodal machine learning and Raman spectroscopy uncover biochemical pathways of autumnal leaf senescence.BMC plant biology · 2026Article
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Characterization of Phyllobilins in Hops: Antioxidant and Potentially Bitter Senescence-Related Metabolites.Journal of agricultural and food chemistry · 2025Article
- Regreening mechanisms in cucumber: insights from a CsSIG2 mutation affecting chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Multi-locus genome-wide association study for grain yield and drought tolerance indices in sorghum accessions.The plant genome · 2024Article
- Identification of Target Gene and Interacting Protein of TwoPlants (Basel, Switzerland) · 2024Article
- Non-additive expression genes play a critical role in leaf vein ratio heterosis in Nicotiana tabacum L.BMC genomics · 2024Article
- Overexpression of MtIPT gene enhanced drought tolerance and delayed leaf senescence of creeping bentgrass (Agrostis stolonifera L.).BMC plant biology · 2024Article
- Wheat NAC transcription factorPlant direct · 2024Article
- Genetic tapestry of Capsicum fruit colors: a comparative analysis of four cultivated species.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Combined BSA-Seq and RNA-Seq Reveal Genes Associated with the Visual Stay-Green of Maize (International journal of molecular sciences · 2023Article
- Ethyl Methane Sulfonate and Sodium Azide-Mediated Chemical and X-ray-Mediated Physical Mutagenesis Positively RegulatePlants (Basel, Switzerland) · 2022Article
- A Chlorophyll-Derived Phylloxanthobilin Is a Potent Antioxidant That Modulates Immunometabolism in Human PBMC.Antioxidants (Basel, Switzerland) · 2022Article
- Side-chain modifications of phyllobilins may not be essential for chlorophyll degradation inPlant direct · 2022Article
- Identification and Defensive Characterization ofInternational journal of molecular sciences · 2022Article
- Multiomics Approach To Decipher the Origin of Chlorophyll Content in Virgin Olive Oil.Journal of agricultural and food chemistry · 2022Article
- Evolution of chlorophyll degradation is associated with plant transition to land.The Plant journal : for cell and molecular biology · 2022Article
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8 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Nonfluorescent chlorophyll catabolites (NCCs) were described as products of chlorophyll breakdown in Arabidopsis thaliana. NCCs are formyloxobilin-type catabolites derived from chlorophyll by oxygenolytic opening of the chlorin macrocycle. These linear tetrapyrroles are generated from their fluorescent chlorophyll catabolite (FCC) precursors by a nonenzymatic isomerization inside the vacuole of senescing cells. Here, we identified a group of distinct dioxobilin-type chlorophyll catabolites (DCCs) as the major breakdown products in wild-type Arabidopsis, representing more than 90% of the chlorophyll of green leaves. The molecular constitution of the most abundant nonfluorescent DCC (NDCC), At-NDCC-1, was determined. We further identified cytochrome P450 monooxygenase CYP89A9 as being responsible for NDCC accumulation in wild-type Arabidopsis; cyp89a9 mutants that are deficient in CYP89A9 function were devoid of NDCCs but accumulated proportionally higher amounts of NCCs. CYP89A9 localized outside the chloroplasts, implying that FCCs occurring in the cytosol might be its natural substrate. Using recombinant CYP89A9, we confirm FCC specificity and show that fluorescent DCCs are the products of the CYP89A9 reaction. Fluorescent DCCs, formed by this enzyme, isomerize to the respective NDCCs in weakly acidic medium, as found in vacuoles. We conclude that CYP89A9 is involved in the formation of dioxobilin-type catabolites of chlorophyll in Arabidopsis.
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