ArticleJournal of mass spectrometry : JMS2013
Direct infusion analysis of nucleotide mixtures of very similar or identical elemental composition.
Article in Journal of mass spectrometry : JMS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Distinguishing Isomeric Cyclobutane Thymidine Dimers by Ion Mobility and Tandem Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2024Article
- Qualitative and Quantitative Analytical Techniques of Nucleic Acid Modification Based on Mass Spectrometry for Biomarker Discovery.International journal of molecular sciences · 2024Review
- Expanding the Epitranscriptomic RNA Sequencing and Modification Mapping Mass Spectrometry Toolbox with Field Asymmetric Waveform Ion Mobility and Electrochemical Elution Liquid Chromatography.Analytical chemistry · 2023Article
- Data-Independent Acquisition for the Detection of Mononucleoside RNA Modifications by Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2022Article
- B-CePs as cross-linking probes for the investigation of RNA higher-order structure.Nucleic acids research · 2021Article
- Recent developments in the characterization of nucleic acids by liquid chromatography, capillary electrophoresis, ion mobility, and mass spectrometry (2010-2020).Journal of separation science · 2021Review
- A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry.Bio-protocol · 2020Article
- Unraveling the RNA modification code with mass spectrometry.Molecular omics · 2020Article
- Collision-Induced Dissociation Studies of Protonated Ions of Alkylated Thymidine and 2'-Deoxyguanosine.Journal of the American Society for Mass Spectrometry · 2020Article
- High-resolution ion mobility spectrometry-mass spectrometry of isomeric/isobaric ribonucleotide variants.Journal of mass spectrometry : JMS · 2020Article
- Direct and Topoisomerase II Mediated DNA Damage by Bis-3-chloropiperidines: The Importance of Being an Earnest G.ChemMedChem · 2017Article
- Global Epitranscriptomics Profiling of RNA Post-Transcriptional Modifications as an Effective Tool for Investigating the Epitranscriptomics of Stress Response.Molecular & cellular proteomics : MCP · 2016Article
- Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells.Nucleic acids research · 2016Article
- Mass spectrometry of modified RNAs: recent developments.The Analyst · 2016Review
- Profiling ribonucleotide modifications at full-transcriptome level: a step toward MS-based epitranscriptomics.RNA (New York, N.Y.) · 2015Article
- Novel RNA modifications in the nervous system: form and function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Review
- The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry.RNA biology · 2014Review
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Authors and funding
4 authors.
Funding
Abstract
The challenges posed by the analysis of mono-nucleotide mixtures by direct infusion electrospray ionization were examined in the context of recent advances of mass spectrometry (MS) technologies. In particular, we evaluated the merits of high-resolution mass analysis, multistep gas-phase dissociation, and ion mobility determinations for the characterization of species with very similar or identical elemental composition. The high resolving power afforded by a linear trap quadrupole-orbitrap allowed the complete differentiation of overlapping isotopic distributions produced by nucleotides that differed by a single mass unit. Resolving (12)C signals from nearly overlapped (13)C contributions provided the exact masses necessary to calculate matching elemental compositions for unambiguous formulae assignment. However, it was the ability to perform sequential steps of gas-phase dissociation (i.e. MS(n)-type analysis) that proved more valuable for discriminating between truly isobaric nucleotides, such as the AMP/dGMP and UMP/ΨMP couples, which were differentiated in the mixture from their unique fragmentation patterns. The identification of diagnostic fragments enabled the deconvolution of dissociation spectra containing the products of coexisting isobars that could not be individually isolated in the mass-selection step. Approaches based on ion mobility spectrometry-MS provided another dimension upon which isobaric nucleotides could be differentiated according to their distinctive mobility behaviors. Subtle structural variations, such as the different positions of an oxygen atom in AMP/dGMP or the glycosidic bond in UMP/ΨMP, produced detectable differences in the respective ion mobility profiles, which enabled the differentiation of the isobaric couples in the mixture. Parallel activation of all ions emerging from the ion mobility element provided an additional dimension for differentiating these analytes on the basis of both mobility and fragmentation properties.
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