ArticleNature protocols2013
Femtosecond optical transfection of individual mammalian cells.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 77 citations in OpenAlex.
- Article
- Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Infrared Laser-Based Single Cell Permeabilization by Plasma Membrane Temperature Gradients.Membranes · 2022Article
- Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration.Frontiers in bioengineering and biotechnology · 2022Review
- Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors.Science advances · 2021Article
- An Overview of Methods and Tools for Transfection of Eukaryotic CellsFrontiers in bioengineering and biotechnology · 2021Review
- Magnetic Force-driven in Situ Selective Intracellular Delivery.Scientific reports · 2018Article
- Investigation of Optimized Treatment Conditions for Acoustic-Transfection Technique for Intracellular Delivery of Macromolecules.Ultrasound in medicine & biology · 2018Article
- Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma.Nature communications · 2017Article
- Oxidative Stress Alters the Morphological Responses of Myoblasts to Single-Site Membrane Photoporation.Cellular and molecular bioengineering · 2017Article
- Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes.Scientific reports · 2016Article
- Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound.Scientific reports · 2016Article
- Nanothermometry: From Microscopy to Thermal Treatments.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Review
- Dynamic manipulation of particles via transformative optofluidic waveguides.Scientific reports · 2015Article
- Software-aided automatic laser optoporation and transfection of cells.Scientific reports · 2015Article
- Cytosolic irradiation of femtosecond laser induces mitochondria-dependent apoptosis-like cell death via intrinsic reactive oxygen cascades.Scientific reports · 2015Article
- Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation.Scientific reports · 2013Article
- Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser-mediated gene transfection into mammalian cells has recently emerged as a powerful alternative to more traditional transfection techniques. In particular, the use of a femtosecond-pulsed laser operating in the near-infrared (NIR) region has been proven to provide single-cell selectivity, localized delivery, low toxicity and consistent performance. This approach can easily be integrated with advanced multimodal live-cell microscopy and micromanipulation techniques. The efficiency of this technique depends on an understanding by the user of both biology and physics. Therefore, in this protocol we discuss the subtleties that apply to both fields, including sample preparation, alignment and calibration of laser optics and their integration into a microscopy platform. The entire protocol takes ~5 d to complete, from the initial setup of the femtosecond optical transfection system to the final stage of fluorescence imaging to assay for successful expression of the gene of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.