ArticleMolecular & cellular proteomics : MCP2013
Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
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- Deep Sequencing MicroRNAs from Extracellular Membrane Vesicles Revealed the Association of the Vesicle Cargo with Cellular Origin.International journal of molecular sciences · 2020Article
- HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKα.Nature communications · 2018Article
- Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy.Biophysical journal · 2017Article
- Role of influenza A virus NP acetylation on viral growth and replication.Nature communications · 2017Article
- The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior.EBioMedicine · 2017Article
- Moss-Produced, Glycosylation-Optimized Human Factor H for Therapeutic Application in Complement Disorders.Journal of the American Society of Nephrology : JASN · 2017Article
- Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.The Journal of experimental medicine · 2015Article
- Quantitative proteogenomic profiling of epidermal barrier formation in vitro.Journal of dermatological science · 2015Article
- Strain Specific Phage Treatment for Staphylococcus aureus Infection Is Influenced by Host Immunity and Site of Infection.PloS one · 2015Article
- Altered MCM protein levels and autophagic flux in aged and systemic sclerosis dermal fibroblasts.The Journal of investigative dermatology · 2014Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratinocytes account for 95% of all cells of the epidermis, the stratified squamous epithelium forming the outer layer of the skin, in which a significant number of skin diseases takes root. Immortalized keratinocyte cell lines are often used as research model systems providing standardized, reproducible, and homogenous biological material. Apart from that, primary human keratinocytes are frequently used for medical studies because the skin provides an important route for drug administration and is readily accessible for biopsies. However, comparability of these cell systems is not known. Cell lines may undergo phenotypic shifts and may differ from the in vivo situation in important aspects. Primary cells, on the other hand, may vary in biological functions depending on gender and age of the donor and localization of the biopsy specimen. Here we employed metabolic labeling in combination with quantitative mass spectrometry-based proteomics to assess A431 and HaCaT cell lines for their suitability as model systems. Compared with cell lines, comprehensive profiling of the primary human keratinocyte proteome with respect to gender, age, and skin localization identified an unexpected high proteomic consistency. The data were analyzed by an improved ontology enrichment analysis workflow designed for the study of global proteomics experiments. It enables a quick, comprehensive and unbiased overview of altered biological phenomena and links experimental data to literature. We guide through our workflow, point out its advantages compared with other methods and apply it to visualize differences of cell lines compared with primary human keratinocytes.
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