ArticleJournal of anatomy2013
Concentration-dependent specimen shrinkage in iodine-enhanced microCT.
Article in Journal of anatomy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 192 citations in OpenAlex.
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- Bioimaging of sense organs and the central nervous system in extant fishes and reptiles in situ: A review.Anatomical record (Hoboken, N.J. : 2007) · 2026Review
- Micro-computed tomography to visualize preserved vascular architecture in decellularized human vaginal tissue: explorative study.Scientific reports · 2025Article
- Evaluating the impact of contrast agents on micro and nano mechanics of soft-to-hard tissue interface.Scientific reports · 2025Article
- Anatomy of the oblique vein of the left atrium: contribution of microCT analysis of human hearts.Surgical and radiologic anatomy : SRA · 2025Article
- Differential performance of aqueous- and ethylic-Lugol's iodine stain to visualize anatomy in μCT-scanned vertebrates.Journal of anatomy · 2025Article
- Contrast-Enhanced Micro-CT Imaging of Murine Mandibles: A Multi-Method Approach for Simultaneous Hard and Soft Tissue Analysis.Journal of clinical periodontology · 2025Article
- The use of ethanol as contrast enhancer in synchrotron X-ray phase-contrast imaging leads to heterogeneous myocardial tissue shrinkage: a case report.Journal of synchrotron radiation · 2025Article
- Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT.Heliyon · 2024Article
- Exploring the anatomy ofInternational journal for parasitology. Parasites and wildlife · 2024Article
- Comparative muscle anatomy of the anuran pelvis and hindlimb in relation to locomotor mode.Journal of anatomy · 2024Article
- Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT.Journal of medical imaging (Bellingham, Wash.) · 2024Article
- Pulmonary vasculature development in congenital diaphragmatic hernia: a novel automated quantitative imaging analysis.Pediatric surgery international · 2024Article
- ChFluids and barriers of the CNS · 2024Article
- Computational Approaches and Observer Variation in the 3D Musculoskeletal Modeling of the Heads ofIntegrative organismal biology (Oxford, England) · 2024Article
- Experimental assessment of diffusible iodine-based contrast-enhanced computed tomography (diceCT) protocols.PeerJ · 2024Article
- Bioimaging marine crustacean brain: quantitative comparison of micro-CT preparations in an Alpheid snapping shrimp.Frontiers in neuroscience · 2024Article
- Post-mortem perinatal imaging: what is the evidence?The British journal of radiology · 2023Review
- Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iodine potassium iodide (I2 KI) solution can be employed as a contrast agent for the visualisation of soft tissue structures in micro-computed tomography studies. This technique provides high resolution images of soft tissue non-destructively but initial studies suggest that the stain can cause substantial specimen shrinkage. The degree of specimen shrinkage, and potential deformation, is an important consideration when using the data for morphological studies. Here we quantify the macroscopic volume changes in mouse skeletal muscle, cardiac muscle and cerebellum as a result of immersion in the common fixatives 10% phosphate-buffered formal saline, 70% ethanol and 3% glutaraldehyde, compared with I2 KI staining solution at concentrations of 2, 6, 10 and 20%. Immersion in the I2 KI solution resulted in dramatic changes of tissue volume, which were far larger than the shrinkage from formalin fixation alone. The degree of macroscopic change was most dependent upon the I2 KI concentration, with severe shrinkage of 70% seen in solutions of 20% I2 KI after 14 days' incubation. When using this technique care needs to be taken to use the lowest concentration that will give adequate contrast to minimise artefacts due to shrinkage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.