ArticleMolecular microbiology2013
Transcriptional regulation of fatty acid biosynthesis in mycobacteria.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Genome-wide transcriptional landscape ofFrontiers in immunology · 2026Article
- The Biosynthetic Pathway of Mycolic Acids: Dual-Function Targets for Tuberculosis Therapeutics and Green Steroid Drugs Biomanufacturing.Pharmaceutics · 2025Review
- Proteomic characterization ofmSystems · 2025Article
- Domain architecture of theHeliyon · 2024Article
- MadR mediates acyl CoA-dependent regulation of mycolic acid desaturation in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mycobacterial fatty acid catabolism is repressed by FdmR to sustain lipogenesis and virulence.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mycobacterium tuberculosis FasR senses long fatty acyl-CoA through a tunnel and a hydrophobic transmission spine.Nature communications · 2020Article
- FasR Regulates Fatty Acid Biosynthesis and Is Essential for Virulence ofFrontiers in microbiology · 2020Article
- Transcriptional Regulator AcrR Increases Ethanol Tolerance through Regulation of Fatty Acid Synthesis inApplied and environmental microbiology · 2019Article
- Path-seq identifies an essential mycolate remodeling program for mycobacterial host adaptation.Molecular systems biology · 2019Article
- Adipocyte Model of Mycobacterium tuberculosis Infection Reveals Differential Availability of Iron to Bacilli in the Lipid-Rich Caseous Environment.Infection and immunity · 2018Article
- Lipid metabolism and its implication in mycobacteria-host interaction.Current opinion in microbiology · 2018Review
- Expression of a recombinant, 4'-Phosphopantetheinylated, active M. tuberculosis fatty acid synthase I in E. coli.PloS one · 2018Article
- Promoter mutagenesis for fine-tuning expression of essential genes in Mycobacterium tuberculosis.Microbial biotechnology · 2018Article
- Role of long-chain acyl-CoAs in the regulation of mycolic acid biosynthesis in mycobacteria.Open biology · 2017Article
- A conditional mutant of the fatty acid synthase unveils unexpected cross talks in mycobacterial lipid metabolism.Open biology · 2017Article
- Analysis of coenzyme A activated compounds in actinomycetes.Applied microbiology and biotechnology · 2016Article
- Mycobacterial Metabolic Syndrome: LprG and Rv1410 Regulate Triacylglyceride Levels, Growth Rate and Virulence in Mycobacterium tuberculosis.PLoS pathogens · 2016Article
- Evolution of Mycolic Acid Biosynthesis Genes and Their Regulation during Starvation in Mycobacterium tuberculosis.Journal of bacteriology · 2015Article
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The main purpose of our study is to understand how mycobacteria exert control over the biosynthesis of their membrane lipids and find out the key components of the regulatory network that control fatty acid biosynthesis at the transcriptional level. In this article we describe the identification and purification of FasR, a transcriptional regulator from Mycobacterium sp. that controls the expression of the fatty acid synthase (fas) and the 4-phosphopantetheinyl transferase (acpS) encoding genes, whose products are involved in the fatty acid and mycolic acid biosynthesis pathways. In vitro studies demonstrated that fas and acpS genes are part of the same transcriptional unit and that FasR specifically binds to three conserved operator sequences present in the fas-acpS promoter region (Pfas). The construction and further characterization of a fasR conditional mutant confirmed that FasR is a transcriptional activator of the fas-acpS operon and that this protein is essential for mycobacteria viability. Furthermore, the combined used of Pfas-lacZ fusions in different fasR backgrounds and electrophoretic mobility shift assays experiments, strongly suggested that long-chain acyl-CoAs are the effector molecules that modulate the affinity of FasR for its DNA binding sequences and therefore the expression of the essential fas-acpS operon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.