ArticleThe Journal of biological chemistry2013
Glycoprotein biosynthesis in a eukaryote lacking the membrane protein Rft1.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG.bioRxiv : the preprint server for biology · 2024Article
- Rft1 catalyzes lipid-linked oligosaccharide translocation across the ER membrane.Nature communications · 2024Article
- Structural and mechanistic studies of the N-glycosylation machinery: from lipid-linked oligosaccharide biosynthesis to glycan transfer.Glycobiology · 2023Review
- Structure and Mechanism of the Lipid Flippase MurJ.Annual review of biochemistry · 2022Review
- Persistence of Trypanosoma brucei as early procyclic forms and social motility are dependent on glycosylphosphatidylinositol transamidase.Molecular microbiology · 2022Article
- Cyclic AMP signalling and glucose metabolism mediate pH taxis by African trypanosomes.Nature communications · 2022Article
- Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry.Scientific reports · 2021Article
- RFT1 Protein Affects Glycosylphosphatidylinositol (GPI) Anchor Glycosylation.The Journal of biological chemistry · 2017Article
- Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.Nature chemical biology · 2016Article
- Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping.Progress in lipid research · 2016Review
- The multiple evolutionary origins of the eukaryotic N-glycosylation pathway.Biology direct · 2016Article
- The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli.PloS one · 2016Article
- "With a Little Help from My Friends"-Social Motility in Trypanosoma brucei.PLoS pathogens · 2015Review
- Flipping a Lipid-Linked Oligosaccharide? You Must Whip It!Trends in biochemical sciences · 2015Article
- A Glycosylation Mutant of Trypanosoma brucei Links Social Motility Defects In Vitro to Impaired Colonization of Tsetse Flies In Vivo.Eukaryotic cell · 2015Article
- Effects of N-glycan precursor length diversity on quality control of protein folding and on protein glycosylation.Seminars in cell & developmental biology · 2015Review
- Lipid Flippases for Bacterial Peptidoglycan Biosynthesis.Lipid insights · 2015Review
- Role of Flippases in Protein Glycosylation in the Endoplasmic Reticulum.Lipid insights · 2015Review
- N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.Microbiology and molecular biology reviews : MMBR · 2014Review
- Comparative Analysis of Protein Glycosylation Pathways in Humans and the Fungal Pathogen Candida albicans.International journal of microbiology · 2014Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Mature dolichol-linked oligosaccharides (mDLOs) needed for eukaryotic protein N-glycosylation are synthesized by a multistep pathway in which the biosynthetic lipid intermediate Man5GlcNAc2-PP-dolichol (M5-DLO) flips from the cytoplasmic to the luminal face of the endoplasmic reticulum. The endoplasmic reticulum membrane protein Rft1 is intimately involved in mDLO biosynthesis. Yeast genetic analyses implicated Rft1 as the M5-DLO flippase, but because biochemical tests challenged this assignment, the function of Rft1 remains obscure. To understand the role of Rft1, we sought to analyze mDLO biosynthesis in vivo in the complete absence of the protein. Rft1 is essential for yeast viability, and no Rft1-null organisms are currently available. Here, we exploited Trypanosoma brucei (Tb), an early diverging eukaryote whose Rft1 homologue functions in yeast. We report that TbRft1-null procyclic trypanosomes grow nearly normally. They have normal steady-state levels of mDLO and significant N-glycosylation, indicating robust M5-DLO flippase activity. Remarkably, the mutant cells have 30-100-fold greater steady-state levels of M5-DLO than wild-type cells. All N-glycans in the TbRft1-null cells originate from mDLO indicating that the M5-DLO excess is not available for glycosylation. These results suggest that rather than facilitating M5-DLO flipping, Rft1 facilitates conversion of M5-DLO to mDLO by another mechanism, possibly by acting as an M5-DLO chaperone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.