ArticleThe Journal of biological chemistry2013
Pentalysine clusters mediate silica targeting of silaffins in Thalassiosira pseudonana.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 68 citations in OpenAlex.
- The Ratio of Silicon to Chitin in Diatom Cell Walls Influences Their Resilience to Environmental Stress.Research (Washington, D.C.) · 2026Article
- ASAFind 2.0: multi-class protein targeting prediction for diatoms and algae with complex plastids.The Plant journal : for cell and molecular biology · 2025Article
- Biohybrid Nanosystem Fabricated with Marine DiatomACS biomaterials science & engineering · 2025Article
- Nanoengineered Silica-Based Biomaterials for Regenerative Medicine.International journal of molecular sciences · 2024Review
- Targeting motifs in frustule-associated proteins from the centric diatomFrontiers in plant science · 2022Article
- Optimizing the Design of Diatom Biosilica-Targeted Fusion Proteins in Biosensor Construction forBiology · 2020Article
- Secondary structure and dynamics study of the intrinsically disordered silica-mineralizing peptide PProteins · 2017Article
- Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica.Scientific reports · 2017Article
- Dynamic responses to silicon in Thalasiossira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs.Scientific reports · 2017Article
- Establishing super-resolution imaging for proteins in diatom biosilica.Scientific reports · 2016Article
- Biochemical Composition and Assembly of Biosilica-associated Insoluble Organic Matrices from the Diatom Thalassiosira pseudonana.The Journal of biological chemistry · 2016Article
- Editing of the urease gene by CRISPR-Cas in the diatomPlant methods · 2016Article
- Targeted drug delivery using genetically engineered diatom biosilica.Nature communications · 2015Article
- Silica Nanowires Templated by Amyloid-like Fibrils.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- Silica Nanowires Templated by Amyloid-like Fibrils.Angewandte Chemie (International ed. in English) · 2015Article
- Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation.Marine drugs · 2015Review
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological formation of inorganic materials (biomineralization) often occurs in specialized intracellular vesicles. Prominent examples are diatoms, a group of single-celled eukaryotic microalgae that produce their SiO2 (silica)-based cell walls within intracellular silica deposition vesicles (SDVs). SDVs contain protein-based organic matrices that control silica formation, resulting in species specifically nanopatterned biosilica, an organic-inorganic composite material. So far no information is available regarding the molecular mechanisms of SDV biogenesis. Here we have investigated by fluorescence microscopy and subcellular membrane fractionation the intracellular transport of silaffin Sil3. Silaffins are a group of phosphoproteins constituting the main components of the organic matrix of diatom biosilica. We demonstrate that the N-terminal signal peptide of Sil3 mediates import into a specific subregion of the endoplasmic reticulum. Additional segments from the mature part of Sil3 are required to reach post-endoplasmic reticulum compartments. Further transport of Sil3 and incorporation into the biosilica (silica targeting) require protein segments that contain a high density of modified lysine residues and phosphoserines. Silica targeting of Sil3 is not dependent on a particular peptide sequence, yet a lysine-rich 12-14-amino acid peptide motif (pentalysine cluster), which is conserved in all silaffins, strongly promotes silica targeting. The results of the present work provide the first insight into the molecular mechanisms for biogenesis of mineral-forming vesicles from an eukaryotic organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.