ArticleThe Journal of biological chemistry2013
Cytoplasmic degradation of the Arabidopsis transcription factor abscisic acid insensitive 5 is mediated by the RING-type E3 ligase KEEP ON GOING.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 128 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Redox-mediated regulation of ABSCISIC ACID-INSENSITIVE 5 affects seed germination and seedling development in Arabidopsis.Journal of experimental botany · 2026Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- Heterologous expression of soybean E3 ubiquitin ligase Glycine max Ring Zinc Finger 1-Like 1 (GmRZFL1) negatively responds to osmotic stress by participating in the abscisic acid signaling pathway in Arabidopsis.BMC plant biology · 2025Article
- QUIRKY controls seed germination via precision degradation of ABI5.Science advances · 2025Article
- Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Dancing molecules: group A bZIPs and PEBPs at the heart of plant development and stress responses.Journal of experimental botany · 2025Review
- The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.The Plant cell · 2024Article
- The RING-Type E3 Ubiquitin Ligase GenePlants (Basel, Switzerland) · 2024Article
- Computational Reconstruction of the Transcription Factor Regulatory Network Induced by Auxin inPlants (Basel, Switzerland) · 2024Article
- A molecular atlas of plastid and mitochondrial proteins reveals organellar remodeling during plant evolutionary transitions from algae to angiosperms.PLoS biology · 2024Article
- CRISPR/Cas9 Gene Editing ofInternational journal of molecular sciences · 2022Article
- ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation.Plant physiology · 2022Article
- COP1 positively regulates ABA signaling during Arabidopsis seedling growth in darkness by mediating ABA-induced ABI5 accumulation.The Plant cell · 2022Article
- HY5 and ABI5 transcription factors physically interact to fine tune light and ABA signaling in Arabidopsis.Plant molecular biology · 2021Article
- Factors that affect protein abundance of a positive regulator of abscisic acid signalling, the basic leucine zipper transcription factor ABRE-binding factor 2 (ABF2).Plant direct · 2021Article
- Control of ABA Signaling and Crosstalk with Other Hormones by the Selective Degradation of Pathway Components.International journal of molecular sciences · 2021Review
- Knockout of the entire family of AITR genes in Arabidopsis leads to enhanced drought and salinity tolerance without fitness costs.BMC plant biology · 2021Article
- SUMO mediated regulation of transcription factors as a mechanism for transducing environmental cues into cellular signaling in plants.Cellular and molecular life sciences : CMLS · 2021Review
- Advances on Post-translational Modifications Involved in Seed Germination.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To mitigate the effects of environmental stress the abscisic acid (ABA)-responsive transcription factor ABI5 is required to delay growth of germinated seedlings. In the absence of stress, KEEP ON GOING (KEG) E3 is required to maintain low levels of ABI5. However, the mechanism underlying KEG-dependent turnover of ABI5 is not known. In addition, localization studies place KEG at the trans-Golgi network, whereas ABI5 is nuclear. Here we show that KEG interacts directly with ABI5 via its conserved C3 region. Interactions between KEG and ABI5 were observed in the cytoplasm and trans-Golgi network only when the RING domain of KEG was inactivated or when ABI5 was stabilized via mutations. Deletion of the C-terminal region of ABI5 or substituting lysine 344 for alanine (K344A) prohibited protein turnover. Furthermore, ABI5 is observed in the cytoplasm of Arabidopsis thaliana root cells when the K344A mutation is combined with the deletion of a nuclear localization signal. Other lysine mutations (K353A, K364A, and K376A) in conjunction with the nuclear localization signal deletion did not result in cytoplasmic accumulation of ABI5. Loss of lysine 344 did not affect the ability of ABI5 to promote ABA responses, which demonstrates that the mutant transcription factor is still functional. Based on the results, a model is suggested where KEG targets ABI5 for degradation in the cytoplasm, thus reducing nuclear accumulation of the transcription factor in the absence of ABA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.