ReviewProtoplasma2013
Protoplasts: a useful research system for plant cell biology, especially dedifferentiation.
Review in Protoplasma, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.).BMC biotechnology · 2026Article
- Dedifferentiation of Plant Cells: A Term Covering Multiple Pathways?Plants (Basel, Switzerland) · 2026Article
- Aggregates-on-chip: a microfluidic device for crowding plant cells in culture.Frontiers in plant science · 2026Article
- Phenotype microarray-based assessment of metabolic variability in plant protoplasts.Plant methods · 2025Article
- Early protoplast culture and partial regeneration inFrontiers in plant science · 2025Article
- Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn.BMC plant biology · 2023Article
- A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture.Micromachines · 2022Article
- A CRISPR/dCas9 toolkit for functional analysis of maize genes.Plant methods · 2020Article
- A Versatile High Throughput Screening Platform for Plant Metabolic Engineering Highlights the Major Role ofFrontiers in plant science · 2020Article
- Evaluation of Methods to AssessFrontiers in plant science · 2019Article
- An Automated Protoplast Transformation System.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Plant stem cells: what we know and what is anticipated.Molecular biology reports · 2018Review
- Genome Editing for Crop Improvement - Applications in Clonally Propagated Polyploids With a Focus on Potato (Frontiers in plant science · 2018Article
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- Article
- A Robotic Platform for High-throughput Protoplast Isolation and Transformation.Journal of visualized experiments : JoVE · 2016Article
- Historical review of research on plant cell dedifferentiation.Journal of plant research · 2015Review
- Article
- Involvement of Plant Stem Cells or Stem Cell-Like Cells in Dedifferentiation.Frontiers in plant science · 2015Review
- An improved high-throughput screening assay for tunicamycin sensitivity in Arabidopsis seedlings.Frontiers in plant science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As protoplasts have the characteristics of no cell walls, rapid population growth, and synchronicity, they are useful tools for research in many fields, especially cellular biology (Table 1). This article is an overview that focuses on the application of protoplasts to investigate the mechanisms of dedifferentiation, including changes in hormone signals, epigenetic changes, and organelle distribution during the dedifferentiation process. The article also emphasizes the wide range of uses for protoplasts in studying protein positions and signaling during different stresses. The examples provided help to show that protoplast systems, for example the mesophyll protoplast system of Arabidopsis, represent promising tools for studying developmental biology. Meanwhile, specific analysis of protoplast, which comes from different tissue, has specific advantages and limitations (Table 2), and it can provide recommendations to use this system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.