ArticleGenome medicine2013
Multiplex target capture with double-stranded DNA probes.
Article in Genome medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02036216 (Detected the Valuable Characteristic Changes Both in Circulating Cell-free DNA and Primary Tumor in the Patients With Hepatocelluar Carcinoma.), which is not on this map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Detected the Valuable Characteristic Changes Both in Circulating Cell-free DNA and Primary Tumor in the Patients With Hepatocelluar Carcinoma.
Who cites it
13 citing papers in PubMed.
- Cost-effective solutions for high-throughput enzymatic DNA methylation sequencing.PLoS genetics · 2025Article
- Utilizing insights of DNA repair machinery to discover MMEJ deletions and novel mechanisms.Nucleic acids research · 2024Article
- An improved molecular inversion probe based targeted sequencing approach for low variant allele frequency.NAR genomics and bioinformatics · 2022Article
- Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology.Biotechnology journal · 2022Article
- Validation of novel forensic DNA markers using multiplex microhaplotype sequencing.Forensic science international. Genetics · 2020Article
- Transplant Virus Detection Using Multiplex Targeted Sequencing.The journal of applied laboratory medicine · 2018Article
- Efficient in situ barcode sequencing using padlock probe-based BaristaSeq.Nucleic acids research · 2018Article
- Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions.Nature biomedical engineering · 2017Article
- Next-Generation Molecular Testing of Newborn Dried Blood Spots for Cystic Fibrosis.The Journal of molecular diagnostics : JMD · 2016Article
- The Bottleneck in Genetic Testing.Circulation research · 2015Article
- A Rapid, High-Quality, Cost-Effective, Comprehensive and Expandable Targeted Next-Generation Sequencing Assay for Inherited Heart Diseases.Circulation research · 2015Article
- microDuMIP: target-enrichment technique for microarray-based duplex molecular inversion probes.Nucleic acids research · 2015Article
- Improved coverage and accuracy with strand-conserving sequence enrichment.Genome medicine · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Target enrichment technologies utilize single-stranded oligonucleotide probes to capture candidate genomic regions from a DNA sample before sequencing. We describe target capture using double-stranded probes, which consist of single-stranded, complementary long padlock probes (cLPPs), each selectively capturing one strand of a genomic target through circularization. Using two probes per target increases sensitivity for variant detection and cLPPs are easily produced by PCR at low cost. Additionally, we introduce an approach for generating capture libraries with uniformly randomized template orientations. This facilitates bidirectional sequencing of both the sense and antisense template strands during one paired-end read, which maximizes target coverage.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.