ArticleAnalytical chemistry2013
Spatially-directed protein identification from tissue sections by top-down LC-MS/MS with electron transfer dissociation.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- The evolving landscape of spatial proteomics technologies in the AI age.Fundamental research · 2026Review
- High-Throughput Proteoform Imaging for Revealing Spatial-Resolved Changes in Brain Tissues Associated with Alzheimer's Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Optimization of Collagenase Proteomics for Improved Mass Spectrometry Imaging Peptide Identification.Analytical chemistry · 2025Article
- Thermal Denaturation of Fresh Frozen Tissue Enhances Mass Spectrometry Detection of Peptides.Analytical chemistry · 2024Article
- Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue.Journal of proteome research · 2023Article
- Article
- Hanging drop sample preparation improves sensitivity of spatial proteomics.Lab on a chip · 2022Article
- Acceleration of age-induced proteolysis in the guinea pig lens nucleus by in vivo exposure to hyperbaric oxygen: A mass spectrometry analysis.Experimental eye research · 2021Article
- Probing protein dissociation from gold nanoparticles and the influence of temperature from the protein corona formation mechanism.RSC advances · 2021Article
- Proteomic characterization of the human lens and Cataractogenesis.Expert review of proteomics · 2021Review
- Spatial proteomics revealed a CXMucosal immunology · 2020Article
- MALDI imaging mass spectrometry of β- and γ-crystallins in the ocular lens.Journal of mass spectrometry : JMS · 2020Article
- A recommended and verified procedure for in situ tryptic digestion of formalin-fixed paraffin-embedded tissues for analysis by matrix-assisted laser desorption/ionization imaging mass spectrometry.Journal of mass spectrometry : JMS · 2019Article
- MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies.Analytical chemistry · 2019Article
- Protein identification strategies in MALDI imaging mass spectrometry: a brief review.Current opinion in chemical biology · 2019Review
- Ambient ionisation mass spectrometry for in situ analysis of intact proteins.Journal of mass spectrometry : JMS · 2018Article
- Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions.Rapid communications in mass spectrometry : RCM · 2018Article
- Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions.Science translational medicine · 2018Article
- Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.Journal of proteome research · 2018Article
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
Corrections and comments
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3 authors.
Funding
Abstract
MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for localizing both small molecules and intact proteins in a wide variety of tissue samples in both normal and diseased states. Identification of imaged signals in MALDI-IMS remains a bottleneck in the analysis and limits the interpretation of underlying biology of tissue specimens. In this work, spatially directed tissue microextraction of intact proteins followed by LC-MS/MS with electron transfer dissociation (ETD) was used to identify proteins from specific locations in three tissue types; ocular lens, brain, and kidney. Detection limits were such that a 1 μL extraction volume was sufficient to deliver proteins to the LC-MS/MS instrumentation with sufficient sensitivity to detect 50-100 proteins in a single experiment. Additionally, multiple modified proteins were identified; including truncated lens proteins that would be difficult to assign to an imaged mass using a bottom-up approach. Protein separation and identification are expected to improve with advances in intact protein fractionation/chromatography and advances in interpretation algorithms leading to increased depth of proteome coverage from distinct tissue locations.
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