ArticlePloS one2013
Exit from competence for genetic transformation in Streptococcus pneumoniae is regulated at multiple levels.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Fever-like temperature bursts promote competence development via an HtrA-dependent pathway in Streptococcus pneumoniae.PLoS genetics · 2023Article
- Identification and Characterization of Negative Regulators of Rgg1518 Quorum Sensing in Streptococcus pneumoniae.Journal of bacteriology · 2023Article
- Investigating theMicrobiology (Reading, England) · 2022Article
- The acquisition of clinically relevant amoxicillin resistance in Streptococcus pneumoniae requires ordered horizontal gene transfer of four loci.PLoS pathogens · 2022Article
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- Competence shut-off by intracellular pheromone degradation in salivarius streptococci.PLoS genetics · 2022Article
- The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The alternative sigma factor σeLife · 2020Article
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- DprA-Dependent Exit from the Competent State Regulates Multifaceted Streptococcus pneumoniae Virulence.Infection and immunity · 2019Article
- Refining the Pneumococcal Competence Regulon by RNA Sequencing.Journal of bacteriology · 2019Article
- Competence beyond Genes: Filling in the Details of the Pneumococcal Competence Transcriptome by a Systems Approach.Journal of bacteriology · 2019Article
- Femtoliter droplet confinement of Streptococcus pneumoniae: bacterial genetic transformation by cell-cell interaction in droplets.Lab on a chip · 2019Article
- Streptococcus endopeptidases promote HPV infection in vitro.MicrobiologyOpen · 2019Article
- Competence inhibition by the XrpA peptide encoded within the comX gene of Streptococcus mutans.Molecular microbiology · 2018Article
- Evolution via recombination: Cell-to-cell contact facilitates larger recombination events in Streptococcus pneumoniae.PLoS genetics · 2018Article
- Dynamic Modeling ofFrontiers in microbiology · 2018Article
- Kinetics of drug-ribosome interactions defines the cidality of macrolide antibiotics.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Unleashing Natural Competence in Lactococcus lactis by Induction of the Competence Regulator ComX.Applied and environmental microbiology · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of natural competence in S. pneumoniae entails coordinated expression of two sets of genes. Early gene expression depends on ComE, a response regulator activated by the pheromone CSP (Competence-Stimulating-Peptide). Subsequently, an early gene product (the alternative sigma factor ComX) activates expression of late genes, establishing the competent state. Expression of both sets of genes is transient, rapidly shut off by a mechanism that depends on the late gene, dprA. It has been thought that the rapid shutoff of late gene expression is the combined result of auto-inhibition of ComE and the instability of ComX. However, this explanation seems incomplete, because of evidence for ComX-dependent repressor(s) that might also be important for shutting off the response to CSP and identifying dprA as such a gene. We screened individual late gene mutants to investigate further the roles of ComX-dependent genes in competence termination. A ΔdprA mutant displayed a prolonged late gene expression pattern, whereas mutants lacking cbpD, cibABC, cglEFG, coiA, ssbB, celAB, cclA, cglABCD, cflAB, or radA, exhibited a wild-type temporal expression pattern. Thus, no other gene than dprA was found to be involved in shutoff. DprA limits the amounts of ComX and another early gene product, ComW, by restriction of early gene expression rather than by promoting proteolysis. To ask if DprA also affects late gene expression, we decoupled late gene expression from early gene regulation. Because DprA did not limit ComX activity under these conditions, we also conclude that ComX activity is limited by another mechanism not involving DprA.
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